2017 Fiscal Year Final Research Report
Development of high-throughput screening methods for antisense oligonucleotides
Project/Area Number |
15K17886
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Research Category |
Grant-in-Aid for Young Scientists (B)
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Allocation Type | Multi-year Fund |
Research Field |
Bio-related chemistry
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Research Institution | Tokyo University of Science |
Principal Investigator |
Hara Rintaro 東京理科大学, 薬学部生命創薬科学科, 助教 (70709766)
|
Project Period (FY) |
2015-04-01 – 2018-03-31
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Keywords | アンチセンス核酸医薬 / リボヌクレアーゼH(RNase H) / スクリーニング / カチオン性オリゴ糖 / カチオン性オリゴペプチド |
Outline of Final Research Achievements |
Antisense oligonucleotides (ASOs) are a promising candidate for nucleic acid drugs. In this study, we aimed to develop high-throughput screening methods in which high-active ASOs are chemically marked. Although we found some functional groups which have appropriate reactivity for these methods, we could not establish the efficient high-throughput screening method. On the other hand, I unintendedly found that some artificial cationic molecules could inhibit the cleavage reaction of mismatched RNAs by ASO and RNase H, which lead directly to side-effects of ASO-type drugs.
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Free Research Field |
生体関連化学
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