Analysis of cartilage-specific IDH mutant mice for explication of underlying mechanism in chondrosarcoma development

2016 Fiscal Year Final Research Report

• Project/Area Number: 15K18398
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Tumor biology
• Research Institution: The University of Tokyo
• Principal Investigator: Hirata Makoto 東京大学, 医科学研究所, 特任助教 (50401071)
• Research Collaborator: Alman Benjamin A.
• Project Period (FY): 2015-04-01 – 2017-03-31
• Keywords: 軟骨腫瘍 / イソクエン酸デヒドロゲナーゼ

Outline of Final Research Achievements

Central chondrosarcomas are known to harbor frequent mutation in IDH1 or IDH2 genes. The objective of the current study is to generate genetically-engineered mice expressing mutant IDH1 or IDH2 specifically in cartilage and to elucidate the underlying mechanism of chondrosarcoma occurrence and development. The mutant IDH1 or IDH2 mice exhibited dwarfism as compared to their wild-type littermate, with morphologically abnormal growth plate cartilage. Of note, the IDH2 mutant mice harbored severer phenotype in cartilage than the IDH1 mutant mice. The mutant IDH1 or IDH2 mice with p53 deficiency were generated by crossing with p53-flox mice. No clear evidence of bone tumor development has been observed until the age of three months old.

Free Research Field

骨軟部腫瘍