2017 Fiscal Year Final Research Report
Molecular basis of cancer therapy targetting the gene amplification
| Project/Area Number |
15K18413
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Tumor biology
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| Research Institution | Kagoshima University |
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Principal Investigator |
MINAMI KENTARO 鹿児島大学, 医歯学総合研究科, 特任研究員 (20735956)
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Keywords | 遺伝子増幅 / 抗癌剤耐性 |
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| Outline of Final Research Achievements |
Although gene amplification is important for tumor development, progression and resistance to anticancer agent, the regulation mechanism of the gene amplification has not been understooed. In this study, we investigated the function and regulation mechanism of BHLHE41. We established doxycycline inducible BHLHE41 expressing cells from cells having RRM1 gene amplification. After treatment doxycycline, RRM1 gene amplification in BHLHE41 expressed cells decreased at 14 days.We established BHLHE41 expressing cells from lung adenocarcinoma cell line A549.These cells grew more slowly than parental cells on dishes and in soft agar.We found that BHLHE41 was targeted for proteasome dependent degradation by ubiquitination.
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| Free Research Field |
分子腫瘍学分野
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