2017 Fiscal Year Final Research Report
Serial femtosecond X-ray crystallography (SFX) of metalloproteins containing light-sensitive chemical structures
| Project/Area Number |
15K18487
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Structural biochemistry
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| Research Institution | Osaka University |
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Principal Investigator |
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Keywords | 量子ビーム科学 / X線結晶構造解析 |
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| Outline of Final Research Achievements |
Copper-containing nitrite reductase (CuNiR) is involved in denitrification of the nitrogen cycle. Synchrotron X-rays rapidly reduce copper sites, induce its catalytic reactions, and decompose the substrate complex structure, which has made crystallographic studies of CuNiR difficult. Using X-ray free electron laser (XFEL) facility, SACLA, we determined intact structures of CuNiR with and without nitrite. Based on the obtained structures, we proposed a redox-coupled proton switch model, which provides a new explanation for proton-coupled electron transfer in CuNiR.
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| Free Research Field |
構造生物学
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