2017 Fiscal Year Final Research Report
The development of the method for determining damage-free crystal structure at room temperature using X-ray free electron laser
| Project/Area Number |
15K18493
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Structural biochemistry
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| Research Institution | Gifu University (2017)
University of Hyogo (2015-2016) |
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Principal Investigator |
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Keywords | プロトンポンプ / 時分割構造解析 / 無損傷構造解析 / X線自由電子レーザー / 膜タンパク質 / 金属タンパク質 |
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| Outline of Final Research Achievements |
Cytochrome c oxidase (CcO), which generates bioenergy by catalyzing O2 for maintain biological activity, is one of the most important protein in bioenergetics field. Understanding the reaction mechanism of CcO is not academically significant subject but also expected to be useful for caring hypoxic diseases. In this study, I succeeded in determining the damage-free CcO crystal structure using X-ray free electron laser which has billion times higher pulse-intensity and one thousandth shorter pulse-width than present X-ray. Furthermore, intermediate structures of CcO photoactivated by laser irradiation were determined by utilizing the extremely short pulse-width of XFEL.
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| Free Research Field |
構造生物学、生化学、生物物理学
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