Qualitative improvement of potato by GrIGS system

2016 Fiscal Year Final Research Report

• Project/Area Number: 15K18624
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Science in genetics and breeding
• Research Institution: Hirosaki University
• Principal Investigator: Kasai Atsushi 弘前大学, 農学生命科学部, 研究員 (80633982)
• Project Period (FY): 2015-04-01 – 2017-03-31
• Keywords: エピゲノム編集 / 新育種技術（NPBT) / 接ぎ木 / 篩管長距離輸送性RNA

Outline of Final Research Achievements

Novel plant breeding technique named as GrIGS (Graft-induced Gene silencing) system, which fully utilize grafting and phloem long-distance transportability of RNA, has been developed. In this study, I tried to apply the GrIGS system to a vegetative propagation crop potato and to obtain epigenome editing plants.
In model experiment using transgene (35S:GFP), specific method of potato was established by modification of GrIGS system. Then I developed this system to endogenous genes to give additional useful trait (suppression of cold-induced sweetening and degradation of amylose contents) for practical use. At least next generation tubers maintained higher methylation level induced by GrIGS system.
Furthermore, I examined the stack, which was achieved by re-grafting with other siRNA donor to target multiple genes and the high methylation level induction method (the second), with same one to increase the methylation level.

Free Research Field

植物分子育種学