Genome editing with CRISPR/Cas system for genome-wide functional genomics in the rice blast fungus

2016 Fiscal Year Final Research Report

• Project/Area Number: 15K18647
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Plant protection science
• Research Institution: Kobe University
• Principal Investigator: Arazoe Takayuki 神戸大学, 科学技術イノベーション研究科, 学術研究員 (40749975)
• Project Period (FY): 2015-04-01 – 2017-03-31
• Keywords: ゲノム編集 / CRISPR/Cas9 / Target-AID / ポストゲノム / イネいもち病菌 / 麹菌 / 網羅的遺伝子破壊 / AID

Outline of Final Research Achievements

Simple and efficient functional gene analysis is a one of the most important issues in the post-genomics era. In this study, we exploited a fungal CRISPR/Cas9 system and applied genome editing to genome-wide functional genomics approaches in the rice blast fungus. Using this system, we established highly efficient gene targeting, multiple gene disruption, base modification, and reporter gene knock-in methods. In addition, we developed a novel genome-editing tool, “Target-AID”, which enables targeted nucleotide substitution without a DNA cleavage and donor gene by cytidine deamination. These genome-editing strategies can be used to expand various fundamental approaches and molecular breeding methods in filamentous fungi.

Free Research Field

植物病理学