Development of a method for distribution analysis of sphingolipids in tissue by mass spectrometry

2016 Fiscal Year Final Research Report

• Project/Area Number: 15K18842
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Physical pharmacy
• Research Institution: Tohoku University
• Principal Investigator: Saigusa Daisuke 東北大学, 東北メディカル・メガバンク機構, 講師 (90545237)
• Project Period (FY): 2015-04-01 – 2017-03-31
• Keywords: スフィンゴ脂質 / レーザーマイクロダイセクション / LC-MS/MS / ヒートスタビライザー / IMS / 質量分析計 / S1P

Outline of Final Research Achievements

In this study, we developed a method for demonstrating tissue distribution of sphingolipids by means of LC-MS/MS and IMS.
We first optimized a time or temperature of heat stabilizer for sphingolipids analysis in tissue. Then, the distribution of sphingolipids in a part of white and red pulp of spleen was demonstrated by LC-MS/MS and IMS, and the concentration of sphingolipids was significantly higher in red pulp than white pulp (P < 0.05). In addition, we demonstrated that the concentration of sphingolipids related with the expression of SPHK or SPL by LC-MS/MS.

Free Research Field

分析化学