2016 Fiscal Year Final Research Report
Imaging mass spectrometry of antibody drugs
| Project/Area Number |
15K19170
|
|---|
| Research Category |
Grant-in-Aid for Young Scientists (B)
|
| Allocation Type | Multi-year Fund |
|---|
| Research Field |
Applied pharmacology
|
|---|
| Research Institution | Osaka University |
|---|
Principal Investigator |
|
|---|
| Project Period (FY) |
2015-04-01 – 2017-03-31
|
| Keywords | 質量分析 / イメージング / 抗体医薬 |
|---|
| Outline of Final Research Achievements |
The main purpose of this research proposal is to visualize the distribution of an antibody drug, Herceptin. As the first step, the target protein was digested with trypsin and specific peptides were detected. However, in the mixture of human plasma and Herceptin, many peptides peaks derived from IgG were detected with high intensity. On the tissue surface, this situation was not improved. Although many peptides derived from abundant proteins such as cytoskeleton were detected, the target peptides were not detected due to ion suppression. Through this research, it is revealed that proposed methodology could not solve the problems to visualize antibody drugs. I consider completely different approaches are essential to achieve the purpose.
|
| Free Research Field |
分析化学
|
