Moyamoya disease mouse model establishment using Rnf213 mutant transgenic mouse

2017 Fiscal Year Final Research Report

• Project/Area Number: 15K19243
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Hygiene and public health
• Research Institution: Chubu University (2017); Kyoto University (2015-2016)
• Principal Investigator: KOBAYASHI Hatasu 中部大学, 生命健康科学部, 准教授 (70542091)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Keywords: もやもや病 / RNF213 / ノックアウトマウス / トランスジェニックマウス

Outline of Final Research Achievements

In this study, we used a vascular endothelial-specific Rnf213 mutant Tg mouse and Rnf213 KO mice to create 1) cerebral hypoxia models using hypoxic chamber and 2) cerebral hypoperfusion model by bilateral common carotid artery stenosis (BCAS) method and evaluated cerebral blood vessels. As a result, it was suggested that angiogenesis was impaired in the vascular endothelial-specific Rnf213 mutant Tg mouse, while arteriogenesis was impaired in in Rnf213 KO mice. These results indicate that the cerebral hypoxia/hypoperfusion model may be useful for establishing a mouse model of Moyamoya disease.

Free Research Field

予防医学