Elucidation of new alimentally cancer disease mechanism of Cullin3 E3 ligase mediated by BTB protein

2017 Fiscal Year Final Research Report

• Project/Area Number: 15K19343
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Gastroenterology
• Research Institution: Nagoya City University
• Principal Investigator: Ozeki Keiji 名古屋市立大学, 大学院医学研究科, 助教 (70750610)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Keywords: 大腸癌 / BTB蛋白 / Cullin3 / ユビキチン / 異所性胃型粘液形質

Outline of Final Research Achievements

BTB protein - Cullin 3 - E 3 ligase has been reported that they may become a new therapeutic target in cancer cells, although there are many unclear points about the function of about BTB proteins. BTB protein (BACK-Kelch protein group) strongly binding to Cullin 3 was identified by using Alpha screening assay. We found a BTB protein (KLHL-X) associated with cell proliferation, due to comprehensively performing SiRNA of BTB protein on colon cancer cell line. Further examinations showed that the expression of KLHL-X might be involved in proliferation and invasion in colorectal cancer.
Next, the relationship of ectopic gastric mucinous phenotype and colorectal lateral spreading tumors (LSTs) are not reported. We analyzed 105 colorectal LSTs to identify factors indicative of malignant transformation and invasion. Ectopic gastric and intestinal phenotype, neuroendocrine cell differentiation, and SOX2 expression differ according to tumor grade on colorectal LSTs.

Free Research Field

cancer