The role of Nicotinamide phosphoribosyltransferase (Nampt) in atrial fibrillation

2016 Fiscal Year Final Research Report

• Project/Area Number: 15K19366
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Cardiovascular medicine
• Research Institution: University of Tsukuba
• Principal Investigator: XU DongZhu 筑波大学, 医学医療系, 助教 (20616651)
• Research Collaborator: Chen Peng-Sheng Indiana university, Krannert institute of Cardiology, 教授 ; Chen Zhenhui Indiana university, Krannert institute of Cardiology, 准教授 ; Murakoshi Nobuyuki 筑波大学, 医学医療系循環器内科, 講師
• Project Period (FY): 2015-04-01 – 2017-03-31
• Keywords: 心房細動 / 肥満 / Nampt / カルシウムハンドリング

Outline of Final Research Achievements

In electrophysiological study, Nampt+/-showed increased AF inducibility (80.0% in Nampt+/-+ND, 44.0% in Nampt+/++ND, p<0.05) and duration compared to Nampt+/+, and HFD accelerated longer duration than ND (15.21±5.75 in Nampt+/-+HFD, 8.83±3.33 in Nampt+/-+ND, 6.17±2.66 in Nampt+/++HFD, 3.39±1.07 in Nampt+/++ND, p<0.05). There was no significant difference in fibrotic area between all group. The gene and protein expression levels of Nampt in the atria were decreased by half in the Nampt+/-, and further decreased in Nampt+/-+HFD. The gene expression levels of calcium handling-related molecules Ryr2 and Atp2a2 were decreased in Nampt+/- compared to those of Nampt+/+. These findings suggested that genetic reduction of Nampt increased AF inducibility and duration partially through modulating calcium handling pathway without obvious structural alterations.

Free Research Field

循環器病態