The establishment of therapeutic strategies of microglial transplantation against chronic ischemic stroke

2017 Fiscal Year Final Research Report

• Project/Area Number: 15K19478
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Neurology
• Research Institution: Niigata University
• Principal Investigator: Kanazawa Masato 新潟大学, 医歯学総合病院, 助教 (80645101)
• Co-Investigator(Renkei-kenkyūsha): Shimohata Takayoshi 岐阜大学, 医学部, 教授 (60361911) ; Takahashi Tetsuya 新潟大学, 医歯学総合病院, 助教 (20515663)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Keywords: 脳梗塞 / ミクログリア / 細胞療法 / 血管新生 / 神経軸策伸展 / M2

Outline of Final Research Achievements

We confirmed primary microglia under oxygen-glucose deprivation (OGD) conditions like mild ischemia polar to protective M2 microglia. We found that intraarterial administration of OGD microglia promoted functional recovery via angiogenesis in the border area within the ischemic core as well as axonal outgrowth in the ischemic penumbra by remodeling factors such as vascular endothelial growth factor (VEGF) and transforming growth factor at 28 days after ischemia. Furthermore, we could isolated primary microglia from adult rats under cerebral ischemia. We also demonstrated that OGD condition prompted more secretion of VEGF than hypoxia or glucose-deprivation stimulus only. In conclusion, intravascular administration of M2 microglia preconditioned by optimal OGD might be a novel therapeutic strategy against ischemic stroke.

Free Research Field

脳血管障害