Research on treatment of Parkinson's disease utilizing optogenetics and iPS cell

2017 Fiscal Year Final Research Report

• Project/Area Number: 15K19498
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Neurology
• Research Institution: Juntendo University
• Principal Investigator: OYAMA GENKO 順天堂大学, 医学部, 准教授 (00407256)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Keywords: iPS / dopamine / controllable / optogenetics

Outline of Final Research Achievements

Fetal cell transplantation for Parkinson's disease did not become a general treatment due to off dyskinesia or tumor formation. Although it is expected to solve the problem by transplanting cells derived from induced pluripotent stem (iPS) cells, its safety and effect are uncertain. In this study, to overcome these problems, we aimed to develop a controllable iPS cell induced dopamine neuron transplantation therapy. Channelrhodopsin 2 was transfected in to iPS cell derived dopamine neuron with Lenti virus. The controllable dopamine neuron was transplanted into striatum of 6-hydroxydopamine dyskinesia model mouse. We confirmed the transplanted cells engrafted without tumorigenesis in the mouse brain and differentiated into dopamine neurons.

Free Research Field

神経内科