2016 Fiscal Year Final Research Report
Pathogenesis of hypoplastic leukemia in murine models
| Project/Area Number |
15K19546
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Hematology
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| Research Institution | The University of Tokyo |
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Principal Investigator |
Arai Shunya 東京大学, 医学部附属病院, 講師 (00579716)
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| Project Period (FY) |
2015-04-01 – 2017-03-31
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| Keywords | Runx1 |
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| Outline of Final Research Achievements |
I transplanted murine hematopoietic progenitor cells (HPCs) transduced with the C-terminus truncated Runx1 mutant as a possible murine model of hypoplastic leukemia. However, the recipient mice have not developed leukemia. Then I constructed several CRISPR/Cas9 vectors that targeted the genomic locus of murine Runx1, and transduced murine HPCs with them. The HPCs expressing truncated Runx1 mutants were assayed by replating in cytokine-rich semisolid culture. In a result, the HPCs with truncated Runx1 mutants have not showed tumorigenic phenotypes such as survival advantage and differentiation block.
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| Free Research Field |
血液内科学
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