Identification of new lipid mediators associated with inflammation in model mice for collagen vascular diseases

2017 Fiscal Year Final Research Report

• Project/Area Number: 15K19577
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Collagenous pathology/Allergology
• Research Institution: Oita University
• Principal Investigator: Ozaki Takashi 大分大学, 医学部, 病院特任助教 (70749374)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Keywords: 脂質メディエーター / SLE / パルミトイルエタノールアミド / オレオイルエタノールアミド / 炎症性サイトカイン / Toll様受容体9

Outline of Final Research Achievements

In this study, we aimed to identify novel lipid mediators which might be associated with inflammation in model mice for collagen vascular diseases. We measured lipid mediators in MRL/lpr mice (lupus model mice) and MRL/MpJ mice (wild type mice) using LC-MS and revealed that PEA and OEA levels were decreased in serum and spleen in MRL/lpr mice compared to MRL/MpJ mice. PEA and OEA inhibited TLR9-mediated production of pro-inflammatory cytokines in bone marrow-derived dendritic cells and B cells in vitro. Moreover, PEA and OEA reduced IL-6 production in mice injected with CpG and D-galactosamine. Further researches are needed to clarify whether PEA and OEA could be potential therapeutic candidates to modulate inflammation in SLE.

Free Research Field

膠原病