2016 Fiscal Year Final Research Report
Analysis of intra- and intertumoral variety of BRAF mutation in Melanoma tumor.
| Project/Area Number |
15K19685
|
|---|
| Research Category |
Grant-in-Aid for Young Scientists (B)
|
| Allocation Type | Multi-year Fund |
|---|
| Research Field |
Dermatology
|
|---|
| Research Institution | Shinshu University |
|---|
Principal Investigator |
|
|---|
| Project Period (FY) |
2015-04-01 – 2017-03-31
|
| Keywords | メラノーマ / ddPCR法 / BRAF変異率 / 部位別定量解析 |
|---|
| Outline of Final Research Achievements |
11 patients who had BRAFV600E-mutated primary lesions detected by Sanger sequencing were enrolled in this study. Further, resected metastatic lesions from 9 patients were also examined. BRAFV600E-mutated rate of primary lesions analyzed by droplet digital PCR (ddPCR) was from 18.5% to 83.3%, and the mutation rate was not varied in each lesion. 3 metastases had low mutation rate as 0.02 to 1.03%, that is, mutation cells were very rare in those metastatic tumors.
These results suggest the possibility that BRAFV600E and BRAFwild melanoma cells do not exist ununiformly, but intermingle in a single melanoma tumor in various ratios.
|
| Free Research Field |
メラノーマ
|
