2017 Fiscal Year Final Research Report
Exploring new combination of radiotherapy and LW6, a novel HIF-1 inhibitor; suprresion of EMT by LW6
| Project/Area Number |
15K19764
|
|---|
| Research Category |
Grant-in-Aid for Young Scientists (B)
|
| Allocation Type | Multi-year Fund |
|---|
| Research Field |
Radiation science
|
|---|
| Research Institution | Hirosaki University |
|---|
Principal Investigator |
Sato Mariko 弘前大学, 医学部附属病院, 助教 (30645263)
|
|---|
| Research Collaborator |
TAKAI Yoshihiro
HIROSE Katsumi
|
|---|
| Project Period (FY) |
2015-04-01 – 2018-03-31
|
| Keywords | 上皮間葉転換（ＥＭＴ） / ＨＩＦ－１ / ＨＩＦ－１阻害剤 / 低酸素 / 放射線治療 |
|---|
| Outline of Final Research Achievements |
Recently study reported that hypoxic cancer cells are promoted epitherial-mesenchymal transition (EMT) by radiation therapy.The induction of hypoxia-induced EMT involvement of HIF-1 pathway has been suggested, therefore it is expected that LW6 improve the radiotherapeutic effect of hypoxic tumor via HIF-1 inhibition.
The human lung adenocarcinoma A549 cells were cultured under normoxia or hypoxia (O2 1%), and X-irradiated 0 or10 Gy. We performed wound-healing assay and evaluated the expression of EMT markers, JNK and p-JNK. In this study, we revealed that LW6 suprress each of hypoxia- and radiation-induced EMT leading to cancer relapse through HIF-1 pathway and JNK pathway blockade.
|
| Free Research Field |
放射線生物学
|
