2017 Fiscal Year Final Research Report
Prognostic significance of circulating tumor cells in patients with gastric cancer: epithelial mesenchymal transition and perioperative kinetics
| Project/Area Number |
15K19841
|
|---|
| Research Category |
Grant-in-Aid for Young Scientists (B)
|
| Allocation Type | Multi-year Fund |
|---|
| Research Field |
General surgery
|
|---|
| Research Institution | Hokkaido University (2016-2017)
Fujita Health University (2015) |
|---|
Principal Investigator |
|
|---|
| Project Period (FY) |
2015-04-01 – 2018-03-31
|
| Keywords | 循環腫瘍細胞 / 上皮間葉転換 / 胃癌 |
|---|
| Outline of Final Research Achievements |
Circulating tumor cells (CTCs) have been shown to be heterogeneous. This study aimed to identify the prognostic significance of CTCs in patients with gastric cancer, focusing on epithelial mesenchymal transition and perioperative kinetics.Peripheral blood was taken from patients (n=54) before curative resection, and at 7 days, 1 and 6 months postoperatively. CTCs were enriched using density gradient centrifugation and magnetic-activated cell sorting (negative selection). Cell suspensions were characterized by multi-immunofluorescence staining against cytokeratin (CK) and N-cadherin, and by DAPI staining. CTCs were defined as nucleated cells expressing CK or N-cadherin. After 2 years, postoperative recurrence was detected in five patients, all of whom had N-cadherin+/CK－/CD45－ CTCs preoperatively.
The recurrence rates were high in the preoperative 1 CTC or more, and increased postoperatively. Perioperative measurement of CTCs may be a useful surrogate marker for recurrence risk.
|
| Free Research Field |
消化器外科
|
