2016 Fiscal Year Final Research Report
Analysis of cancer-specific urinary exosomal miRNA derived from urothelial carcinoma
| Project/Area Number |
15K20089
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Urology
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| Research Institution | Osaka University |
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Principal Investigator |
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| Project Period (FY) |
2015-04-01 – 2017-03-31
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| Keywords | 尿路上皮癌 / エクソソーム / 尿中 / バイオマーカー |
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| Outline of Final Research Achievements |
Exosomes were extracted from urine of 6 urothelial carcinoma (UC) patients and 3 healthy volunteers by ultracentrifugation, and miRNA from urinary Exosomes was analyzed by miRNA array. We identified 5 miRNAs (miR-155-5p, miR-15a-5p, miR-21-5p, miR-132-3p and miR-31-5p) overexpressed in urinary exosomes of UC patients from miRNA microarray analysis.Five candidate miRNAs were all significantly expressed more highly in urinary exosomes of UC patients compared to those of the control in verification cohort (N=60, all P<0.0001). Of the 5 miRNAs, miR-21-5p in urinary exosomes was the most potent biomarker, having a sensitivity of 75.0%, specificity of 95.8%. This result was better than that of urine cytology in this cohort.
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| Free Research Field |
泌尿器科腫瘍
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