2017 Fiscal Year Final Research Report
The study of the phospholipid metabolizing enzymes and endoplasmic reticulum in the inner ear.
| Project/Area Number |
15K20178
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Otorhinolaryngology
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| Research Institution | Yamagata University |
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Principal Investigator |
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| Research Collaborator |
KAKEHATA SEIJI 山形大学, 医学部, 教授 (60594322)
GOTO KAORU 山形大学, 医学部, 教授 (30234975)
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Keywords | 内耳 / リン脂質代謝酵素 / ジアシルグリセロールキナーゼ / 小胞体 |
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| Outline of Final Research Achievements |
The results of RT-PCR of DGK isozymes revealed that DGKζ, DGKε and DGKα were strongly expressed in the cochlea of normal guinea pigs. DGKζ was strongly detected in the nucleus of inner hair cells and supporting cells, but only weakly found in nuclei of outer hair cells. DGKε plasmids with FLAG tag were prepared, immunoprecipitated, silver stained, and the detected proteins were submitted to mass spectrometry. However, DGKε was not found to bind to protein in the endoplasmic reticulum in this study.
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| Free Research Field |
耳科学
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