Role of Local Immunoglobulin E Production in the Pathophysiology of Eosinophilic Chronic Rhinosinusitis

2017 Fiscal Year Final Research Report

• Project/Area Number: 15K20201
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Otorhinolaryngology
• Research Institution: Osaka University
• Principal Investigator: Takeda Kazuya 大阪大学, 医学部附属病院, 医員 (90734054)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Keywords: 好酸球性副鼻腔炎 / IgE / 鼻腔細菌 / Th2細胞 / クラススイッチ / 鼻茸

Outline of Final Research Achievements

We generated monoclonal antibodies from IgE+ plasmablasts of nasal polyps(NPs) of ECRS patients. In the reactivity tests, we found that ~20% of expressed antibodies reacted with nasal bacteria. In vitro T cell stimulation assay with identified antigens revealed that patients had more bacteria-reactive Th2 cells than healthy individuals. High-throughput sequencing analysis unraveled that local IgEs were originated from polyp-residing IgG+ and/or IgA+ B cells. These results suggest that protective mucosal immunity against nasal bacteria might convert into allergic inflammation through IgE class-switching in ECRS patients.

Free Research Field

耳鼻咽喉科学