The role of osteopontin in reparative dentinogenesis

2016 Fiscal Year Final Research Report

• Project/Area Number: 15K20359
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Morphological basic dentistry
• Research Institution: Niigata University
• Principal Investigator: SAITO Kotaro 新潟大学, 医歯学系, 助教 (10733719)
• Project Period (FY): 2015-04-01 – 2017-03-31
• Keywords: 象牙芽細胞 / オステオポンチン / 窩洞形成 / 修復象牙質 / マウス

Outline of Final Research Achievements

This study aimed to clarify the role of OPN during reparative dentinogenesis.In WT mice, reparative dentin formation continued next to the preexisting dentin at the mesial coronal pulp. In contrast, there was no reparative dentin in the Opn KO mice where newly differentiated odontoblast-like cells lacked immunoreaction for type I collagen. The in vitro organ culture demonstrated that the administration of recombinant OPN rescued the type I collagen secretion by odontoblast-like cells in the Opn KO mice. The results suggested that the deposition of OPN at the calcification front is essential for the type I collagen secretion by newly differentiated odontoblast-like cells to form reparative dentin during pulpal healing following cavity preparation.

Free Research Field

口腔解剖学