2016 Fiscal Year Final Research Report
Development of new predictive factors for malignant alteration of oral cancer by the functional analysis of the factors in regard to loss of anchorage dependence.
| Project/Area Number |
15K20534
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Surgical dentistry
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| Research Institution | Yamaguchi University |
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Principal Investigator |
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| Research Collaborator |
HARADA Koji 山口大学, 大学院医学系研究科, 助教 (60253217)
FERDOUS Tarannum 山口大学, 大学院医学系研究科, 学術研究員 (70647932)
KURAMITSU Yasuhiro 山口大学, 大学院医学系研究科, 准教授 (50281811)
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| Project Period (FY) |
2015-04-01 – 2017-03-31
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| Keywords | Calreticulin / 口腔癌 / 足場依存性喪失 / プロテオミクス |
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| Outline of Final Research Achievements |
We used QR-32 cells in this study which is a regressive murine fibrosarcoma cell clone; and QRsP-11, a progressive malignant tumor cell clone originated from QR-32. CALR is a Ca2+-binding protein. This study was aimed to determine the usefulness of CALR as a prognostic factor in patients with oral cancers. Our findings suggest that elevated expression of CALR might play an important role in tumor progression in oral cancer, and could be considered as a useful prognostic factor in oral cancer patients. Next, we tried to investigate the functions of CALR in oral squamous cell carcinoma cell lines by using CALR-siRNA. We transfected CALR-siRNA into HSC2 and HSC3 cells. Down-regulation of CALR resulted in growth inhibition and lower cell migration on HSC2 and HSC3. These results suggest that down-regulation of CALR expressions might conductive to the reduction of OSCC malignancy.Our data suggested that CALR may be a new predictive factor for malignant alteration of oral cancer.
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| Free Research Field |
口腔癌
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