Development of control system for periodontopathic biofilm using SspB peptide

2017 Fiscal Year Final Research Report

• Project/Area Number: 15K20610
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Orthodontics/Pediatric dentistry
• Research Institution: Nihon University
• Principal Investigator: ITO Tatsuro 日本大学, 松戸歯学部, 講師 (60635126)
• Research Collaborator: SENPUKU Hidenobu 国立感染症研究所, 細菌第一部第六室, 室長 (20250186)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Keywords: 小児歯科学 / バイオフィルム / SspBペプチド

Outline of Final Research Achievements

To understand the three-way interaction among S. gordonii, P. gingivalis and salivary gp340 as a unit, we established a peptide binding assay using SspB (390-T400K-402). P. gingivalis 33277 showed the highest binding activity of the tested bacteria, whereas P. gingivalis W83, which was deficient in Mfa1 fimbriae, exhibited poor binding activity, as did S. gordonii. The binding of SspB (390-T400K-402) peptide in saliva- or gp340-treated P. gingivalis was significantly higher than that in non-treated cells. The SspB (390-T400K-402) peptide binding assay revealed that initial attachment of P. gingivalis to the substrata of S. gordonii may require gp340-mediated SspB-Mfa1 interactions. The assay is available to assess the relationships among SspB, Mfa1 and salivary gp340 as a unit. Taken together, a peptide binding assay using SspB (390-T400K-402) presented herein may provide important insights into the development of control system for periodontopathic biofilm.

Free Research Field

矯正・小児系歯学