Novel oxidative stress response mechanism by reactive sulfur species and 8-nitro-cGMP

2017 Fiscal Year Final Research Report

• Project/Area Number: 15K20876
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Pathological medical chemistry; Chemical biology
• Research Institution: Tohoku University
• Principal Investigator: Tomoaki Ida 東北大学, 医学系研究科, 助教 (70570406)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Keywords: 活性イオウ分子種 / 8-ニトロ-cGMP / システインパースルフィド / 酸化ストレス / 質量分析

Outline of Final Research Achievements

We investigated novel oxidative stress response mechanism by 8-nitro-cGMP and reactive sulfur species such as cysteine persulfide. We developed a quantitative method, i.e, sulfur omics analysis, for determination of the level of cysteine persulfides/polysulfides and protein polysulfidation using liquid chromatography-tandem mass spectrometry (LC-MS/MS) and iodoacetamide-based derivative. Using this technique, it was found that reactive sulfur species play an important role in oxidative stress response, via crosstalk with 8-nitro-cGMP. We also found a new cysteine persulfide producing system in cells, which is originally known as a master enzyme for protein translation, and elucidated its pivotal roles in protein polysulfidation and protein function.

Free Research Field

分析科学