Development of a microfluidic well plate for CTC detection

2016 Fiscal Year Final Research Report

• Project/Area Number: 15K20933
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Biomedical engineering/Biomaterial science and engineering; Medical systems
• Research Institution: The University of Tokyo
• Principal Investigator: Kaneda Shohei 東京大学, 生産技術研究所, 助教 (10542467)
• Research Collaborator: FUJII Teruo ; COLLARD Dominique ; LAGADEC Chann ; MEIGNAN Samuel
• Project Period (FY): 2015-04-01 – 2017-03-31
• Keywords: バイオ関連機器 / がん / マイクロ・ナノデバイス / マイクロ流体デバイス

Outline of Final Research Achievements

In this project, a microfluidic well plate to separate circulating tumor cell (CTC) from peripheral blood were developed. The microfluidic well plate has 96 wells for CTC separation and each well connected to a single outlet via a microfluidic network. Different kinds of cell analysis against separated CTCs can be independently performed depending on each well.　In evaluation tests, separation of PC-3 cells (prostate cancer cell line) spiked into peripheral blood obtained from healthy donor, and following viability assay using fluorescent stain (Calcein AM and Propidium Iodide) and immunofluorescence (FITC labeled anti-EpCAM antibody) had been successfully performed on the plate.

Free Research Field

応用マイクロ流体システム