Proteomic analysis of the failing human heart by mass spectrometry

2016 Fiscal Year Final Research Report

• Project/Area Number: 15K20942
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Human pathology; Laboratory medicine
• Research Institution: The University of Tokyo
• Principal Investigator: Domoto Yukako (新谷裕加子) 東京大学, 医学部附属病院, 特任講師 (30596961)
• Project Period (FY): 2015-04-01 – 2017-03-31
• Keywords: 心臓 / プロテオミクス

Outline of Final Research Achievements

A left ventricular assist device (LVAD) is used for patients with heart failure as a bridge to heart transplantation. To reveal the molecular markers that are associated with an improvement of cardiac function and myocardial remodeling, the myocardial tissue from patients with heart failure was analyzed with liquid-chromatography mass spectrometry. As the first step to gain the candidates of prognostic markers, we tried finding the myocardial proteins in which change was observed following LVAD support. For each patient, we quantified at least 1,000 different kinds of protein in this study. This proteomics approach suggests that mitochondrial proteins are convincing markers to characterize the molecular changes in myocardium during the course of LVAD support.

Free Research Field

心臓血管病理、腎病理