Clarification of the mechanism of platelet hypofunction caused by extracorporeal circulation-assist devices using next-generation sequencing

2016 Fiscal Year Final Research Report

• Project/Area Number: 15K21287
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Emergency medicine; Anesthesiology
• Research Institution: Kyoto Prefectural University of Medicine
• Principal Investigator: Sachiko Maeda 京都府立医科大学, 医学(系)研究科(研究院), 助教 (90529512)
• Project Period (FY): 2015-04-01 – 2017-03-31
• Keywords: 血小板 / microRNA

Outline of Final Research Achievements

Extracorporeal circulation-assist devices are known to induce platelet activation and hypofunction, and cause complications such as thromboembolism and coagulopathy. The present study was aimed to identify microRNAs in platelets associated with platelet activation and hypofunction caused by extracorporeal circulation-assist devices. We compared microRNA expression changes in platelets between pre- and post-cardiopulmonary bypass (CPB) using next-generation sequencing technology. Up to the present, we have analyzed 20 patients receiving cardiac surgeries under CPB, and identified several significant microRNA alterations (including hypoxia-inducible microRNAs) between pre- and post-CPB. Focusing on these microRNAs, we will perform in vitro studies as the next step.

Free Research Field

麻酔科学