Appropriate deoxyribonucleosides for measuring bacterial production from the viewpoint of DNA synthesis rate

2016 Fiscal Year Final Research Report

• Project/Area Number: 15K21449
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Aquatic bioproduction science; Environmental dynamic analysis
• Research Institution: Soka University
• Principal Investigator: Tsuchiya Kenji 創価大学, 理工学部, 助教 (70739276)
• Research Collaborator: SANO Tomoharu 国立環境研究所, 環境計測研究センター, 主任研究員 (10178808) ; TOMIOKA Noriko 国立環境研究所, 地域環境研究センター, 主任研究員 (40168399) ; SHIMODE Shinji 横浜国立大学, 環境情報研究院, 准教授 (70397090)
• Project Period (FY): 2015-04-01 – 2017-03-31
• Keywords: バクテリア生産 / 安定同位体 / LC-MS / DNA合成 / デオキシリボヌクレオシド

Outline of Final Research Achievements

Incorporation characteristics of four deoxyribonucleosides (thymidine, deoxyadenosine, deoxyguanosine and deoxycytidine) composing DNA in natural bacterial communities were examined to evaluate appropriate deoxyribonucleosides for measuring bacterial production from the viewpoint of DNA synthesis rate. The highest incorporation rate among the four deoxyribonucleosides was deoxyguanosine. The present study suggests that measurement of bacterial production by using deoxyguanosine possibly reflects larger numbers of bacterial species productions.

Free Research Field

生物海洋学