Differentiation potential of osteoblast from cultured C2C12 cells on zirconia disk.

2016 Fiscal Year Final Research Report

• Project/Area Number: 15K21457
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Dental engineering/Regenerative dentistry; Prosthodontics/ Dental materials science and
• Research Institution: Tsurumi University
• Principal Investigator: SAITO Mari 鶴見大学, 歯学部, 学部助手 (60739332)
• Project Period (FY): 2015-04-01 – 2017-03-31
• Keywords: 歯周組織再生 / 歯根膜付きインプラント / 細胞増殖分化 / 成長因子 / TGF-β / BMP / オッセオインテグレーション / ジルコニア

Outline of Final Research Achievements

This study approached from a variety of angles which focus on interface of the dental implants and the alveolar bone. New knowledge was obtained it about growth factors affect the proliferation and differentiation of mesenchymal cells.
1. Six human periodontal ligament (HPDL) cell lines were picked up, TGF-β and BMP regulated osteoblastic differentiation of all cell lines. 2. Intracellular TGF-β was activated by MMPs enhanced in HPDL cells by Er:YAG laser irradiation. 3. We identified TGF-β isoforms in porcine immature and mature enamel of developing teeth. These results suggest that TGF-β1 is predominant in the secretory-stage enamel, while both TGF-β1 and β3 coexist in the maturation-stage enamel. 4. We established cell lines from porcine dental pulp and examined the effects of BMP2 and TGF-β on the odontoblastic differentiation. After differentiation, odontoblastic cells were expressed the gene of DSPP and MMP-20.

Free Research Field

生化学　分子生物学　歯周病学　歯科インプラント学