2016 Fiscal Year Final Research Report
Development of artificial cartilage-bone substitute by dynamic culture and two-layered scaffold
| Project/Area Number |
15K21563
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Prosthodontics/ Dental materials science and
Dental engineering/Regenerative dentistry
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| Research Institution | Fukuoka Dental College |
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Principal Investigator |
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| Project Period (FY) |
2015-04-01 – 2017-03-31
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| Keywords | 力学刺激 / 組織工学 / scaffold / コラーゲン / β-TCP / 幹細胞 / 3次元培養 |
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| Outline of Final Research Achievements |
In the present study, a multi-phase porous composite scaffold consisting of β-tricalcium phosphate (β-TCP) and collagen was developed for bone tissue engineering. Rat bone marrow mesenchymal stem cells (rMSC) were cultured in collagen/β-TCP or collagen scaffold for up to 7 days in order to assess the effect of the mechanical stimulus on cell proliferation and differentiation. It was demonstrated that the porous collagen/β-TCP scaffold and collagen scaffold possessed a continuous porous structure with a pore size ranging to 200μm. In both cases, the cell number increased up to 7 days compared with the static culture. The ALP activity clearly increased in proportion to the period of cell culture compared with the results for static culture. It was concluded that the mechanical stimulus effectively promotes cell growth and osteoblast differentiation.
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| Free Research Field |
生体工学
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