The molecular pathogenesis of immune tolerance and host defense in primary human placenta-derived trophoblasts

2016 Fiscal Year Final Research Report

• Project/Area Number: 15K21655
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Obstetrics and gynecology; Embryonic/Neonatal medicine
• Research Institution: National Center for Child Health and Development
• Principal Investigator: Motomura Kenichiro 国立研究開発法人国立成育医療研究センター, 免疫アレルギー・感染研究部, 共同研究員 (00724329)
• Project Period (FY): 2015-04-01 – 2017-03-31
• Keywords: 合胞体栄養膜細胞 / 胎盤 / 妊娠合併症 / Toll like receptors

Outline of Final Research Achievements

Term human placenta derived primary cultured cytotrophoblasts (differentiated CTB: dCTB) predominantly expressed innate immune receptors for double stranded RNA. Stimulation with poly I:C (synthetic double stranded RNA) induced production of anti-viral cytokines and immune regulatory molecules in dCTB. Moreover, Stimulation with polyI:C decreased dCTB survival rate; pan-caspase inhibitor abrogated the cell death, suggesting that the dCTB cell death by polyI:C is likely to be apoptosis via mitochondria pathway.
Further studies about the relationship between these phenomena and pregnancy complications will possibly elucidate the unknown etiology of pregnancy complications.

Free Research Field

妊娠と免疫