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2008 Fiscal Year Final Research Report

Regulation of Bio-nanosystems

Research Project

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Project/Area Number 16083101
Research Category

Grant-in-Aid for Scientific Research on Priority Areas

Allocation TypeSingle-year Grants
Review Section Biological Sciences
Research InstitutionThe University of Tokyo (2008-2009)
Tohoku University (2004-2007)

Principal Investigator

HIGUCHI Hideo  The University of Tokyo, 大学院・理学系研究科, 教授 (90165093)

Co-Investigator(Kenkyū-buntansha) SHINGYOJI Chikako  東京大学, 大学院・理学系研究科, 准教授 (80125997)
UCHIDA Takafumi  東北大学, 大学院・農学研究科, 教授 (80312239)
HIROSE Kenzo  東京大学, 大学院・医学系研究科, 教授 (00292730)
SUTOH Kazuo  東京大学, 大学院・総合文化研究科, 教授 (20111453)
Project Period (FY) 2004 – 2009
Keywordsナノシステム / ダイニン / ミオシン / キネシン / 細胞
Research Abstract

The aim of a general group is that the group members determine the goal of our project and promote research work to achieve the goal. To reach the aim, we opened the annual meetings for all project members to present their works. After the meetings, we discussed the problems for reaching the goal and the plan to do. We also had international symposiums in the middle of the project and at the final project year to discuss our works internationally.

  • Research Products

    (44 results)

All 2010 2009 2008 2007 2006 2005 2004 Other

All Journal Article (17 results) Presentation (15 results) Book (5 results) Remarks (1 results) Patent(Industrial Property Rights) (6 results)

  • [Journal Article] In Vivo Nano-imaging of Membrane Dynamics in Metastatic Tumor Cells Using Quantum Dots2010

    • Author(s)
      K Gonda, T M. Watanabe N. Ohuchi, *H. Higuchi
    • Journal Title

      J. Biol. chem 285

      Pages: 2750-2757

  • [Journal Article] Systematic comparison of In vitro motile properties between chlamydomonas wild-type and mutant outer arm dyneins each lacking one of the three heavy chain2009

    • Author(s)
      Furuta A., T. Yagi, H. Yanagisawa, H. Higuchi, *R. Kamiya
    • Journal Title

      J. Biol. Chem 284

      Pages: 5927-5935

  • [Journal Article] Intracellular imaging of targeted proteins la-beled with Quantum Dots2008

    • Author(s)
      Yoo J., T. Kambara, K. Gonda, *H. Higuchi
    • Journal Title

      Exp Cell Res 314

      Pages: 3563-3569

  • [Journal Article] Single-Molecule Visualization of Diffusion at the Solution-Crystal Interface2008

    • Author(s)
      Sazaki, G., M. Okada, T. Matsui, T. Watanabe, H. Higuchi, K. Tsukamoto, K. Nakajima
    • Journal Title

      Crtstal Growth and Design 8

      Pages: 2024-2031

  • [Journal Article] Human Myosin Vc Is a Low Duty Ratio Nonprocessive Motor2008

    • Author(s)
      Watanabe S., T.M. Watanabe, O. Sato, J. Awata, K. Homma, N. Umeki, H. Higuchi, R. Ikebe, *M. Ikebe
    • Journal Title

      J. Biol. Chem 283

      Pages: 10581-10592

  • [Journal Article] Three-dimensional nanometry of vesicle transport in a living cell using dual-focus imaging optics2007

    • Author(s)
      Watanabe, T.M., T. Sato, K. Gonda, *H. Higuchi
    • Journal Title

      Biochem. Biophys. Res. Comm. In press

  • [Journal Article] Stepwise Movements in Vesicle Transport of HER2 by Motor Proteins in Living Cells2007

    • Author(s)
      Watanabe, T.M., *H. Higuchi
    • Journal Title

      Biophysical J. 92

      Pages: 1-12

  • [Journal Article] In vivo Real-time Tracking of Single Quantum Dots Conjugated with Monoclonal Anti-HER2 Antibody in Tumors of Mice2007

    • Author(s)
      H. Tada, *H. Higuchi, T.M. Wanatabe, N. Ohuchi
    • Journal Title

      Cancer Res. 67

      Pages: 1138-44

  • [Journal Article] Reduction in nonfluorescence state of quantum dots on an immunofluorescence staining2006

    • Author(s)
      S. Li-Shishido, T.M. Watanabe, H. Tada, *H. Higuchi, N. Ohuchi
    • Journal Title

      Biochem. Biophys. Res. Comm. 351

      Pages: 7-13

  • [Journal Article] Overlapping hand-over-hand mechanism of single molecular motility of cytoplasmic dynein2006

    • Author(s)
      S. Toba, T.M. Watanabe, L-Y Okimoto, Y.Y. Toyoshima, *H. Higuchi
    • Journal Title

      Proc. Natl. Acad. Sci. USA. 103

      Pages: 5741-45

  • [Journal Article] Single-Molecule Visuarization of Enviroment-Sensitive Fluorophores Inserted into cell membranes by Staphylococcal gamma-hemolysin2006

    • Author(s)
      A.H. Nguyen, V.T. Nguyen, Y. Kamio, H. Higuchi.
    • Journal Title

      Biochemistry 45

      Pages: 2570-6

  • [Journal Article] Biased Binding of Single Molecules and Continuous Movement Of Multiple Molecules of Truncated Single-Headed Kinesin2005

    • Author(s)
      Takashi Kamei, Seiji Kakuta, *Hideo Higuchi
    • Journal Title

      Biophysical Journal 88

      Pages: 2068-77

  • [Journal Article] Motility of myosin V regulated by the dissociation of single calmodulin2005

    • Author(s)
      Nguyen HoaAnh, *Hideo Higuchi
    • Journal Title

      Nature Struct Mol Biol 12

      Pages: 127-132

  • [Journal Article] W177 and R198, of LukF for Phosphatidylcholine-Binding and Pore-Formation by Staphylococcal {gamma}-Hemolysin on Human Erythrocyte Membranes2004

    • Author(s)
      Monma N, Nguyen VT, Kaneko J, Higuchi H, *Kamio Y. Essential Residues
    • Journal Title

      J Biochem (Tokyo). 136

      Pages: 427-431

  • [Journal Article] Mechanochemical coupling of two substeps in a single myosin V motor2004

    • Author(s)
      Uemura S, Higuchi H, Olivares AO, De La Cruz EM, *Ishiwata S
    • Journal Title

      Nat Struct Mol Biol 11

      Pages: 877-83

  • [Journal Article] Rapid double 8-nm steps by a kinesin mutant2004

    • Author(s)
      Higuchi H, Bronner CE, Park HW, *Endow SA
    • Journal Title

      EMBO J 23

      Pages: 2993-9

  • [Journal Article] Regional rheological differences in locomoting neutrophils2004

    • Author(s)
      Yanai, M.J.P. Butler, T. Suzuki. H. Sasaki, H. Higuchi
    • Journal Title

      Am. J. Physiol 287

      Pages: 603-611

  • [Presentation] Cargo Transport by Single Molecular Motors2009

    • Author(s)
      Higuchi, H.
    • Organizer
      The Biophysical Society's 53rd Annual Meeting
    • Place of Presentation
      Boston, USA.
    • Year and Date
      20090200
  • [Presentation] Motility of single molecules of dynein, kinesin, and myosin in vitro and in cells2009

    • Author(s)
      Higuchi, H
    • Organizer
      Symposium on the MESO CONTROL of the cells, by the cells, for the cells.
    • Place of Presentation
      Kyoto, Japan.
    • Year and Date
      20090100
  • [Presentation] Molecular mechanism of dynein motility in vitro and cells2008

    • Author(s)
      Higuchi, H.
    • Organizer
      The 46th Annual Meeting of the Biophysical Society of Japan
    • Place of Presentation
      Fukuoka, Japan.
    • Year and Date
      20081200
  • [Presentation] Imaging of stepwise motility of single motor molecules in living cells2008

    • Author(s)
      Higuchi, H
    • Organizer
      39th NIPS International Symposium & 7th OIB Symposium
    • Place of Presentation
      Okazaki, Japan
    • Year and Date
      20081100
  • [Presentation] Myosin, kinesin and dynein in vitro and cells2008

    • Author(s)
      Higuchi, H.
    • Organizer
      nd International Symposium on Bio-nanosystems
    • Place of Presentation
      Tokyo, Japan
    • Year and Date
      20081000
  • [Presentation] Step size and force generated by motor protein dynein2008

    • Author(s)
      Higuchi, H
    • Organizer
      236th National Meeting & Exposition of the American Chemical Society
    • Place of Presentation
      Philadelphia, USA
    • Year and Date
      20080800
  • [Presentation] Step size of dynein is determined by distances of a power stroke and fluctuation2008

    • Author(s)
      Higuchi, H
    • Organizer
      Gordon Reserch Conferences
    • Place of Presentation
      New Hampshire, USA
    • Year and Date
      20080600
  • [Presentation] Single molecule imaging of motor proteins in vitro and in vivo2008

    • Author(s)
      Higuchi, H
    • Organizer
      Bio-Soft Matter Workshop
    • Place of Presentation
      Tokyo, Japan
    • Year and Date
      20080600
  • [Presentation] Step size and force generated by axonemal and cytoplasmic dynein2006

    • Author(s)
      Higuchi, H
    • Organizer
      Biophysical society Discussion meeting
    • Place of Presentation
      Alisoma, USA
    • Year and Date
      20061000
  • [Presentation] Single molecule imaging of motor proteins in living cells2006

    • Author(s)
      Higuchi, H., T.M. Watanabe
    • Organizer
      The 7th international conference on systems Biology
    • Place of Presentation
      Yokohama, Japan
    • Year and Date
      20061000
  • [Presentation] Introduction to the motor proteins2006

    • Author(s)
      Higuchi, H.
    • Organizer
      International symposium on Bio-nanosystems
    • Place of Presentation
      Mastushima, Japan
    • Year and Date
      20060900
  • [Presentation] Coordination of two single-headed cytoplasmic dyneins creates processive move-ment2006

    • Author(s)
      Higuchi, H.
    • Organizer
      Gordon Research Conference
    • Place of Presentation
      New Hampshire, USA
    • Year and Date
      20060600
  • [Presentation] Nano-biology and nano-medicine on can-cer cells2005

    • Author(s)
      Higuchi, H., T.M. Watanabe, H. Tada, N. Ohuchi
    • Organizer
      Nano-Science and Technology for Medical Applications.
    • Place of Presentation
      Sendai, Japan
    • Year and Date
      20050200
  • [Presentation] Nano-biology and nano-medicine on can-cer cells2005

    • Author(s)
      Higuchi, H., T.M. Watanabe, H. Tada, N. Ohuchi
    • Organizer
      Nano-Science and Technology for Medical Applications
    • Place of Presentation
      Sendai, Japan
    • Year and Date
      20050200
  • [Presentation] Biased Binding, Processive Steps and Oscillatory Movement of Single-headed Kinesin2004

    • Author(s)
      Higuchi, H
    • Organizer
      Structural Insights into Kinesin Function
    • Place of Presentation
      Washington, USA
    • Year and Date
      20041200
  • [Book] 生命現象の動的理解を目指すライブイメージング(ナノメディシン)2008

    • Author(s)
      樋口秀男
    • Total Pages
      125-131
    • Publisher
      羊土社
  • [Book] 単一量子ドットのバイオ・医療ナノイメージング(ナノメディシン)2008

    • Author(s)
      樋口秀男
    • Total Pages
      52-63
    • Publisher
      Ohmsha
  • [Book] 量子ドットを用いたがん細胞の単一分子イメージング(量子ドットの生命科学領域への応用)2007

    • Author(s)
      樋口秀男
    • Total Pages
      176-185
    • Publisher
      シーエムシー出版
  • [Book] 生体分子モーター(非侵襲・可視化技術ハンドブック)2007

    • Author(s)
      樋口秀男
    • Total Pages
      732-737
    • Publisher
      NTS出版
  • [Book] 分子モーター概論(生物物理学ハンドブック)2007

    • Author(s)
      樋口秀男
    • Total Pages
      340-342
    • Publisher
      朝倉書店
  • [Remarks]

    • URL

      http://nanobio.phys.s.u-tokyo.ac.jp/NanoSystem/

  • [Patent(Industrial Property Rights)] 光学装置及び結像方法2006

    • Inventor(s)
      渡邉朋信, 樋口秀男
    • Industrial Property Rights Holder
      渡邉朋信, 樋口秀男
    • Industrial Property Number
      特許権2006-112946
    • Acquisition Date
      2006-04-17
  • [Patent(Industrial Property Rights)] 光学装置2005

    • Inventor(s)
      渡邉朋信, 樋口秀男, 佐藤崇
    • Industrial Property Rights Holder
      渡邉朋信, 樋口秀男, 佐藤崇
    • Industrial Property Number
      特許権2005-336613
    • Acquisition Date
      2005-11-22
  • [Patent(Industrial Property Rights)] 位置解析方法及び位置解析装置2005

    • Inventor(s)
      渡邉朋信, 樋口秀男, 佐藤崇
    • Industrial Property Rights Holder
      渡邉朋信, 樋口秀男
    • Industrial Property Number
      特許権2005-307917
    • Acquisition Date
      2005-10-24
  • [Patent(Industrial Property Rights)] がん細胞への選択的作用組成物および治療用レーザー照射装置2005

    • Inventor(s)
      渡邉朋信, 樋口秀男, 佐藤崇
    • Industrial Property Rights Holder
      渡邉朋信, 樋口秀男
    • Industrial Property Number
      特許権2005-163399
    • Acquisition Date
      2005-06-03
  • [Patent(Industrial Property Rights)] 顕微鏡ステージ、および、焦点位置計測装置と共焦点顕微鏡システム2005

    • Inventor(s)
      渡邉朋信, 樋口秀男, 佐藤崇
    • Industrial Property Rights Holder
      渡邉朋信, 樋口秀男
    • Industrial Property Number
      特許権2005-128871
    • Acquisition Date
      2005-04-27
  • [Patent(Industrial Property Rights)] 単粒子三次元位置追跡方法2004

    • Inventor(s)
      渡邉朋信, 樋口秀男, 佐藤崇
    • Industrial Property Rights Holder
      渡邉朋信, 樋口秀男
    • Industrial Property Number
      特許権2004-303004
    • Acquisition Date
      2004-10-18

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Published: 2011-06-18   Modified: 2016-04-21  

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