The assembly mechanism and infectivity of the Double-layered virus particles

2009 Fiscal Year Final Research Report

• Project/Area Number: 16087209
• Research Category: Grant-in-Aid for Scientific Research on Priority Areas
• Allocation Type: Single-year Grants
• Review Section: Biological Sciences
• Research Institution: National Agricultural Research Organization
• Principal Investigator: OOMURA Toshihiro National Agricultural Research Organization, 中央農研・病害虫検出同定法研究チーム, 専門員 (20355499)
• Co-Investigator(Kenkyū-buntansha): ICHIKI Tamaki 農研機構, 中央農研, 主任研究員 (70355501)
• Project Period (FY): 2004 – 2009
• Keywords: 生体超分子 / ウイルス / タンパク質 / 分子認識 / 構造構築機構

Research Abstract

X-ray crystallographic, cryoelectron microscopic, electron tomographic studies of isolated Rice dwarf virus particles, its component proteins and ultra-thin sections of virus-infected tissues, combined with in vivo studies using virus-infected insect vector cells revealed the mechanism of viral entry into cells, packaging of all the 12 genome segments and 7 component proteins, functions and roles of each of 12 viral proteins during viral assembly in viro, interaction between virus and host factors, viral transfer of intra-inter-cells, and viral exit from infected cells.

Research Products

• [Journal Article] The functional organization of the internal components of Rice dwarf virus (2010)
  • Author(s): Miyazaki, N., Wu, B., Hagiwara, K., Wang, C.Y., Xing, L., Hammer L., Higashiura, A., Tsukihara, T., Nakagawa, A., Omura, T., Cheng, H
  • Journal Title: The Journal of Biochemistry (in press)
  • Peer Reviewed
• [Journal Article] Associationof Rice gall dwarf virus with microtubules is necessary for viral release from cultured insect vector cells (2009)
  • Author(s): Wei, T., Uehara-Ichiki, T., Miyazaki, N., Hibino, H., Iwasaki, K., Omura, T
  • Journal Title: Journal of Virology Pages: 10830-10835
  • Peer Reviewed
• [Journal Article] Endomembranes and myoshin mediate assembly into tubules of Pns10 of Rice dwarf virus and intercellular spreading of the virus in cultured insect vector cells (2008)
  • Author(s): Wei, T., Shimizu, T., Omura, T
  • Journal Title: Virology 372 Pages: 349-356
  • Peer Reviewed
• [Journal Article] Rice dwarf virus is engulfed into and released via vesicular compartments in cultured inset vector cells (2008)
  • Author(s): Wei, T., Hibino, H., Omura, T
  • Journal Title: Journal of General Virology 89(11) Pages: 2915-2920
  • Peer Reviewed
• [Journal Article] Pleomorphic configuration of the trimeric capsid proteins of rice dwarf virus that allows formation of both the outer capsid and tubular crystals (2008)
  • Author(s): Iwasaki, K., Miyazaki, N., Hammer, L., Zhu, Y., Omura, T., Wu, B., Sjoborg, F., Yonekura, K., Murata, K., Namba, K., Caspar, D.L., Fujiyoshi, Y., Cheng, R. H
  • Journal Title: Journal of Molecular Biology 383(1) Pages: 252-265
  • Peer Reviewed
• [Journal Article] Structural evolution of Reoviridae revealed by Oryzavirus in acquiring the second capsid shell (2008)
  • Author(s): Miyazaki, N., Uehara-Ichiki, T., Xing, L., Bergman, L, Higashiura, A., Nakagawa, A., Omura, T., Cheng, R. H
  • Journal Title: Journal of Virology 82(22) Pages: 11344-11353
  • Peer Reviewed
• [Journal Article] Molecular analysis of the genome segments S1, S4, S6 S7 and S12 of a Rice gall dwarf virus isolate from Thailand; completion of the genomic sequence (2007)
  • Author(s): Moriyasu, Y., W. Maruyama-Funatsuki, A. Kikuchi, K. Ichimi, B. Zhong, J. Yan, Y. Zhu, H. Suga, Y. Watanabe, T. Ichiki-Uehara, T. Shimizu, K. Hagiwara, H. Kamiunten, K. Akutsu, T. Omura
  • Journal Title: Archives of Virology 152 Pages: 1315-1322
  • Peer Reviewed
• [Journal Article] Entry of Rice dwarf virus into cultured cells of its insect vector involves clathrin-mediated endocytosis (2007)
  • Author(s): Wei, T., Chen, H., Ichiki-Uehara, T., Hibino, H., Omura, T
  • Journal Title: Journal of Virology 81 Pages: 7811-7815
  • Peer Reviewed
• [Journal Article] The P2 capsid protein of the noenveloped rice dwarf phytoreovirus induces membrane fusion in insect host cells (2007)
  • Author(s): Zhou, F., Pu, Y., Wei, T., Liu, H., Deng, W., Wei, C., Ding, B., Omura, T., Li, Y
  • Journal Title: Proceedings of National Academy of Sciences of USA 104 Pages: 19547-19552
  • Peer Reviewed
• [Journal Article] Three-dimensional architecture of virus-packed tubule (2007)
  • Author(s): Katayama, S., Wei, T., Omura, T., Takagi, J., Iwakaki, K
  • Journal Title: Journal of Electron Microscopy 56(3) Pages: 77-81
  • Peer Reviewed
• [Journal Article] The Pns 12 proteinof Rice dwarf virus is essential for formation of ivroplasms and nucleationof viral assembly complexes (2006)
  • Author(s): Wei, T., Shimizu, T., Hagiwara, K., Kikuchi, A., Moriyasu, Y., Suzuki N., Chen, H., Omura, T
  • Journal Title: Journal of General Virology 87 Pages: 429-438
  • Peer Reviewed
• [Journal Article] ore particles by the outer capsid P8 protein of Rice dwarf virus expressed in transgenic rice plants (2006)
  • Author(s): Hagiwara, K., Higashi, T., Takahashi, K., Hara, N., Aoki, H., Miyazaki N., Wang, Q-Y., Zhu, Y., Yatou, O., Tanaka, H., Omura, T
  • Journal Title: Journal of General Plant Patholgoy 72 Pages: 186-189
  • Peer Reviewed
• [Journal Article] Pns4 of Rice dwarf virus is a phosphoprotein, localized around the viroplasm matrix and forms minitubules (2006)
  • Author(s): Wei, T., Kikuchi, A., Suzuki, N., Shimizu, T., Hagiwara, K., Chen, H., Omura, T
  • Journal Title: Archives of Virology 151 Pages: 1701-1712
  • Peer Reviewed
• [Journal Article] The spread of Rice dwarf virus among cells of its insect vector exploits virus-induced tubular structures (2006)
  • Author(s): Wei, T., Kikuchi, A., Moriyasu, Y., Suzuki, N., Shimizu, T., Hagiwara, K., Chen, H., Takahashi, M., Ichiki-Uehara, T., Omura, T
  • Journal Title: Journal of Virology 80 Pages: 8593-8602
  • Peer Reviewed
• [Journal Article] Transcapsidation and the Conserved Interactions of Two Major Structural Proteins of a Pair of Phytoreoviruses Confirm the Mechanism of Assembly of the Outer Capsid Layer (2005)
  • Author(s): Miyazaki, N., Hagiwara, K., Naitow, H., Higashi, T., Cheng, R., Tsukihara, T., Nakagawa, A., Omura, T
  • Journal Title: J. Mol. Biol 345 Pages: 229-237
  • Peer Reviewed
• [Journal Article] The Amino-terminal region of major capsid protein P3 is essential for self-assembly of single-shelled core-like particles of Rice dwarf virus (2004)
  • Author(s): Hagiwara, K., Higashi, T., Miyazaki, N., Naitow, H., Cheng, H., Nakagawa, A., Mizuno, H., Tsukihara, T., Omura, T
  • Journal Title: Journal of Virology 78 Pages: 3145-3149
  • Peer Reviewed
• [Presentation] 植物レオウイルスがコードするバイロプラズマタンパク質のX線結晶構造解析 (2009)
  • Author(s): 秋田総理、東浦彰史、北尾雅博、清水巧、一木珠樹、笹谷孝英、鈴木守、月原冨武、中川敦史、大村敏博
  • Organizer: 特定領域研究「生体超分子構造」第6回公開シンポジウム
  • Place of Presentation: 豊中市千里ライフサイエンスセンター
  • Year and Date: 20091201-20091202
• [Presentation] 媒介昆虫細胞内におけるRice gall dwarf virusのミトコンドリアへの局在 (2009)
  • Author(s): 清水巧、Taiyun Wei、宮崎直幸、一木(植原)珠樹、日比野啓行、岩崎憲治、大村敏博
  • Organizer: 第57回日本ウイルス学会学術集会
  • Place of Presentation: 東京都都市センターホテル
  • Year and Date: 20091025-20091027
• [Presentation] Rice gall dwarf virusは、マイクロチューブルを介して昆虫細胞内を移行し細胞外に放出される (2009)
  • Author(s): 一木(植原)珠樹、ウエイタイユン、宮崎直幸、日比野啓行、岩崎憲治、大村敏博
  • Organizer: 第57回日本ウイルス学会学術集会
  • Place of Presentation: 東京都都市センターホテル
  • Year and Date: 20091025-20091027
• [Presentation] イネ黒条萎縮ウイルス由来バイロプラズマタンパク質のx線結晶構造解析 (2009)
  • Author(s): 秋田総理、東浦彰史、Pu Yingying、清水巧、一木珠樹、笹谷孝英、鈴木守、中川敦史、大村敏博
  • Organizer: 生体超分子の構造解析と機能制御の原子機構第5回ワークショップ
  • Place of Presentation: 湘南国際村センター
  • Year and Date: 20090727-20090729
• [Presentation] 微小管とRice gall dwarf virus (2009)
  • Author(s): 岩崎憲治、宮崎直幸、Taiyun Wei、一木珠樹、日比野啓行、大村敏博
  • Organizer: 生体超分子の構造解析と機能制御の原子機構第5回ワークショップ
  • Place of Presentation: 湘南国際村センター
  • Year and Date: 20090727-20090729
• [Presentation] イネ黒条萎縮ウイルス由来バイロプラズマタンパク質のx線結晶構造解析 (2009)
  • Author(s): 秋田総理、東浦彰史、Pu Yingying、清水巧、一木珠樹、笹谷孝英、鈴木守、中川敦史、大村敏博
  • Organizer: 第9回日本蛋白質科学会年会
  • Place of Presentation: 京都市国際会議場
  • Year and Date: 20090520-20090522
• [Presentation] イネ縞葉枯ウイルス由来タンパク質p4のx線結晶構造解析とその機能解析 (2009)
  • Author(s): 東浦彰史、篠原智子、秋田総理、清水巧、一木珠樹、鈴木守、笹谷孝英、大村敏博、中川敦史
  • Organizer: 第9回日本蛋白質科学会年会
  • Place of Presentation: 京都市国際会議場
  • Year and Date: 20090520-20090522
• [Presentation] 特定領域研究「生体超分子構造」 (2009)
  • Author(s): 大村敏博. 二重殻ウイルスの複製機構
  • Organizer: 第6回公開シンポジウム
  • Place of Presentation: 豊中市千里ライフサイエンスセンター
  • Year and Date: 2009-12-01
• [Book] 顕微鏡(イネ萎縮ウイルスのライフサイクルにおける超分子複合体の解析) (2009)
  • Author(s): 岩崎憲治、宮崎直幸、片山寿美枝、大村敏博
  • Total Pages: 82-86
• [Book] 日本結晶学会誌(ファイトレオウイルスの2層タンパク質構造の構築機構(MS36)) (2009)
  • Author(s): 大村敏博
  • Total Pages: 3-74
• [Book] VIRUS REPORT.(クライオ電子顕微鏡単粒子解析法を用いたウイルス構造解析) (2009)
  • Author(s): 宮崎直幸、岩崎憲治、大村敏博
  • Total Pages: 52-61
• [Book] 平成20年度年報(イネ萎縮ウイルスの昆虫細胞における伝搬機構の解明) (2009)
  • Author(s): 片山寿美枝、宮崎直幸、Taiyun Wei、日比野啓行、大村敏博、岩崎憲治
  • Total Pages: 41-44
  • Publisher: 大阪大学超高圧電子顕微鏡センター
• [Book] Structure-based study of viral replication(The assembly of the double-layered capsids of phytoreoviruses.) (2007)
  • Author(s): Omura, T., Miyazaki, N., Naitow, H., Cheng, R.H., Tsukihara, T., Nakagawa, A
  • Total Pages: 463-482
  • Publisher: World Scientific.