2006 Fiscal Year Final Research Report Summary
Analysis of different processes of gene transfer in aquatic ecosystems using the Aquatron
| Project/Area Number |
16207001
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| Research Category |
Grant-in-Aid for Scientific Research (A)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Ecology/Environment
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| Research Institution | Research Institute for Humanity and Nature (2005-2006)
Kyoto University (2004) |
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Principal Investigator |
KAWABATA Zen'ichiro Research Institute for Humanity and Nature, Research Department, Professor, 研究部, 教授 (80108456)
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| Co-Investigator(Kenkyū-buntansha) |
NASU Masao Osaka University, Graduate School of Pharmacology, Professor, 大学院薬学研究科, 教授 (90218040)
ENDO Ginro Tohokugakuin University, Faculty of Engineering, Professor, 工学部, 教授 (80194033)
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| Project Period (FY) |
2004 – 2006
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| Keywords | Aquatron / Horizontal gene transfer / Natural transformation / Conjugation / Transduction / Microcystis aeruginosa bloom / Transposition / Phage |
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| Research Abstract |
1 Bacterial DNAs which were released from their cells by phage infection are potential resources for gene transfer by natural transformation. Number of bacteria which took a dissolved plasmid with GFP gene in their cells by natural transformation is 100 times as great as that of bacteria which expressed GFP gene. This indicates natural transformation might take place in natural environments with a higher frequency than we have regarded so far.
2 Tetrahymena thermophila, a bacteriovore, inhibited a conjugal transfer from Escherichia coli to Pseudomonas stutzeri by predation on them, and the coexistence of T. thermophila with Microcystis aeruginosa, a producer, enhanced the inhibition.
3 A mini tranposon (mini Tn) was constructed by the transposon Tn MERI1 coding the mercury resistance genes. The mini Tn was introduced into a non-conjugal plasmid. Then this plasmid was transferred into Eshericia coli DH1with conjugal plasmid R338. It was observed the mini Tn transposed into R338 and then the mini Tn on R338 was transferred to Eshericia coli HB101 by conjugation. This suggested the possibility a conjugal transfer through transposition among replicon in a bacterial cell.
4 DNA sequences of the Tn MERI1 type transposon, IR and DR reiterated sequences on mercury resistance bacterial chromosome were analyzed. This analyses revealed that worldwide gene transfer by transposon took place among bacteria.
5 A community dominated with Microcystis aeruginosa, cyanobacteria, in the Aquatron reduced an infectivity of phage EC 10.
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Research Products
(28 results)
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[Journal Article] Diversity of virus-like agents killing Microcystis aeruginosa in a hyper-eutrophic pond.2006
Author(s)
Honjo, M., Matsui, K., Ueki, M., Nakamura, R., Fuhrman, J.A., Kawabata, Z.
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Journal Title
Journal of Plankton Research 28
Pages: 407-412
Description
「研究成果報告書概要(和文)」より
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[Journal Article] Dissemination of TnMERI1-like mercury resistance transposons among Bacillus isolated from worldwide environmental samples.2004
Author(s)
Narita, M., Matsui, K., Ishii, H., Huang, C.-C., Kawabata, Z., Endo, G.
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Journal Title
FEMS Microbiology Ecology 48(1)
Pages: 47-55
Description
「研究成果報告書概要(和文)」より
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