2007 Fiscal Year Final Research Report Summary
Regenerative medicine for musclo-skeltal system using autologous mesenchymal stem cell conjugated with magnet beads or magnet liposome
| Project/Area Number |
16209045
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| Research Category |
Grant-in-Aid for Scientific Research (A)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Orthopaedic surgery
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| Research Institution | Hiroshima University |
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Principal Investigator |
OCHI Mitsuo Hiroshima University, Hospital, Professor (70177244)
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| Co-Investigator(Kenkyū-buntansha) |
YASUNAGA Yuji Hiroshima University, Graduate School of Biomedical Sciences, Visiting Professor (40253075)
ADACHI Nobuo Hiroshima University, Graduate School of Biomedical Sciences, Assistant Professor (30294383)
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| Project Period (FY) |
2004 – 2007
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| Keywords | regenerative medicine / mesenchymal stem cell / magnetic force / external magnetic device / cell delivery system |
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| Research Abstract |
The purpose of this research project was to establish new cell delivery system using magnetically labeled cells and external magnetic device heading for future clinical application. We have used several different cells such as mesenchymal stem cells (MSC) from bone marrow, neural progenitor cells, or natural killer cells as cell source.
First, we have established methods to label those cells magnetically using liposome, magnetic beads, or Ferumoxides ((Fe_2O_3)_n (FeO)_m). We also made an external magnetic device, which can be used for human clinical application.
As for in vivo study for cartilage regeneration using this cell delivery system, we made osteochondral defects in the swine patella and injected magnetically labeled MSC under influence of external magnet force. We confirmed that the injected magnetically labeled cells were accumulated to the cartilage defect of the patella against gravity even 90 minutes after magnetic force application. In vitro study, we accumulated those magnetically labeled MSC on the degenerated cartilage piece taken from osteoarthritic knee using this cell delivery system. Then, those accumulated cells attached to the cartilage piece were cultured for 3 weeks in chondrogenic differentiation medium. After 3 weeks of culture, cartilage-like layers were formed on the degenerated cartilage piece indicating cartilage regeneration using this cell delivery system.
Beside several researches for cartilage regeneration, we have established cell delivery system for spinal cord injury, bone defect, or malignant tumor. The results of those researches were all positive and were published in international journals, which have high impact factors.
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Research Products
(206 results)
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[Journal Article] A novel cell delivery system using magnetically labeled mesenchymal stem cells and an external magnetic device for clinical cartilage repair2008
Author(s)
Takaaki, Kobayashi, Mistuo, Ochi, Shinobu, Yanada, Masakazu, Ishikawa, Nobuo, Adachi, Masataka, Deie, Koji, Arihiro
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Journal Title
Arthroscopy 24(1)
Pages: 69-76
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Accumulation of magnetically labeled rat mesenchymal stem cells using an external magnetic force, and their potential for bone regeneration2008
Author(s)
Toshihiro, Sugioka, Mitsuo, Ochi, Yuji, Yasunaga, Nobuo, Adachi, Shinobu, Yanada
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Journal Title
J Biomed Mater Res 85(3)
Pages: 597-604
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Magnetically labeled neural progenitor cells, which are localized by magnetic force, promote axon growth in organotypic cocultures2007
Author(s)
Takahiko, Hamasaki, Nobuhiro, Tanaka, Naosuke, Kamei, Osamu, Ishida, Shinobu, Yanada, Kazuyoshi, Nakanishi, Koji, Nishida, Yoshiaki, Oishi, Seiichi, Kawamata, Norio, Sakai, Mistuo, Ochi
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Journal Title
Spine 32(21)
Pages: 2300-2305
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Effects of CD44 antibody-or RGDS peptide-immobilized magnetic beads on cell proliferation and chondrogenesis of mesenchymal stem cells2006
Author(s)
Shinobu, Yanada, Mitsuo, Ochi, Nobuo, Adachi, Hiroo, Nobuto, Muhammad, Agung, Seiichi, Kawamata
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Journal Title
Journal of Biomedical Materials Research 77(4)
Pages: 773-784
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Magnetic targeting of bone marrow stromal cells into spinal cord : through cerebrospinal fluid2006
Author(s)
Koji, Nishida, Nobuhiro, Tanaka, Kazuyoshi, Nakanishi, Naosuke, Kamei, Takahiko, Hamasaki, Shinobu, Yanada, Yu, Mochizuki, Mitsuo, Ochi
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Journal Title
Neuroreport 17(12)
Pages: 1269-1272
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Characterization of labeled neural progenitor cells for magnetic targeting2005
Author(s)
Takahiko, Hamasaki, Nobuhiro, Tanaka, Osamu, Ishida, Shinobu, Yanada, Naosuke, Kamei, Yasushi, Fujiwara, Koji, Nishida, Kazuyoshi, Nakanishi, Patrick, Sharman, Seiichi, Kawamata, Mitsuo, Ochi
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Journal Title
Neuroreport 16(15)
Pages: 1641-1645
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Magnetically labeled human natural killer cells, accumulated in vitro by an external magnetic force, are effective against HOS osteosarcoma cells2005
Author(s)
Yuko, Nakashima, Masataka, Deie, Shinobu, Yanada, Patrick, Sharman, Mitsuo, Ochi
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Journal Title
International Journal of Oncology 27(4)
Pages: 965-971
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] In vitro cartilage formation using TGF-B-immobilized magnetic beads and mesenchymal stem cell-magnetic bead complexes under magnetic field conditions
Author(s)
Mitsuru, Motoyama, Masataka, Deie, Atsushi, Kanaya, Makoto, Nishimori, Ayato, Miyamoto, Shinobu, Yanada, Nobuo, Adachi, Mitsuo, Ochi
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Journal Title
J Biomed Mater Res A (in press)
Description
「研究成果報告書概要(欧文)」より
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[Presentation] Implantation of tissue-engineered cartilage made ex vivo for the treatment of full-thickness cartilage defects of the knee. Follow-up study with 10 years clinical experience2007
Author(s)
Nobuo, Adachi, Mitsuo, Ochi, Masataka, Deie, Masakazu, Ishikawa, Yohei, Ito
Organizer
International Society of Arthroscopy, Knee Surgery and Orthopaedic Sports Medicine
Place of Presentation
Florence, Italy
Year and Date
20070527-31
Description
「研究成果報告書概要(欧文)」より
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[Presentation] 変形性関節症の治療2007
Author(s)
越智光夫
Organizer
日赤集談会及び学術講演会
Place of Presentation
庄原市
Year and Date
2007-12-13
Description
「研究成果報告書概要(和文)」より
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[Presentation] 変形性関節症治療の最前線2007
Author(s)
越智光夫
Organizer
「見える・聞こえる・歩けるように」議員連盟勉強会
Place of Presentation
東京都
Year and Date
2007-09-18
Description
「研究成果報告書概要(和文)」より
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[Presentation] Magnetically labeled neural progenitor cells, which are localized by magnetic force, promote corticospinal axon growth in organotypic co-cultures-New cell delivery system using magnetic targeting2007
Author(s)
Takahiko, Hamasaki, Nobuhiro, Tanaka, Naosuke, Kamei, Kazuyoshi, Nakanishi, Koji, Nishida, Mitsuo, Ochi
Organizer
53rd Annual Meeting of Orthopaedic Research Society
Place of Presentation
San Diego, USA
Year and Date
2007-02-11
Description
「研究成果報告書概要(欧文)」より
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[Presentation] Magnetically labeled neural progenitor cells, which are localized by magnetic force, promote corticospinal axon growth in organotypic co-cultures-New cell delivery system using magnetic targeting2006
Author(s)
Takahiko, Hamasaki, Nobuhiro, Tanaka, Naosuke, Kainei, Kazuyoshi, Nakanishi, Koji, Nishida, Mitsuo, Ochi
Organizer
21st North American Spine Society Annual Meeting
Place of Presentation
Seattle, USA
Year and Date
2006-09-26
Description
「研究成果報告書概要(欧文)」より
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[Presentation] Novel Cell Delivery System using Magnetically Labeled Mesenchymal Stem Cell and External Magnetic Device fm Articular Cartilage Defect2006
Author(s)
Takaaki, Kobayashi, Mitsuo, Ochi, Shinobu, Yanada, Satoshi, Ujigo, Nobuo, Adachi
Organizer
4th Congress of Asia-Pacific Knee Society
Place of Presentation
Seoul, Korea
Year and Date
2006-09-22
Description
「研究成果報告書概要(欧文)」より
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[Presentation] 軟骨再生2006
Author(s)
越智光夫
Organizer
第17回広島整形外科先端医学セミナー
Place of Presentation
広島市
Year and Date
2006-06-12
Description
「研究成果報告書概要(和文)」より
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