2007 Fiscal Year Final Research Report Summary
An analysis of the cell intrinsic timer in oligodendrocyte development
| Project/Area Number |
16300109
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Neuroscience in general
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| Research Institution | The University of Tokyo (2005-2007)
Foundation for Biomedical Research and Innovation (2004) |
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Principal Investigator |
TOKUMOTO Yasuhito The University of Tokyo, Graduate School of Engineering, Lecturer (70261170)
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| Co-Investigator(Kenkyū-buntansha) |
FUJIKAWA Hirokazu Hyogo College of Medicine, Faculty of Medicine, Lecturer (40312136)
YAMAMOTO Hideyuki Hyogo College of Medicine, Faculty of Medicine, Associat Professor (70373529)
MOKAWA Yutaka Fukuoka Prefectural University, 看護学部, Associat Professor (90373274)
KANEMURA Yonehiro Advanced Industrial Science and Technology, Research Institute of Cell Engeneering, Investigator (80344175)
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| Project Period (FY) |
2004 – 2007
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| Keywords | Oligodendrocyte / Development / Bioloeical timer / Brain / Thyroid Hormone |
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| Research Abstract |
In mammalian development, the timing of terminal differentiation of each cell lineage is very important. It determines the total number of cell division and, as a result, determines the body size, the body shape, the organ function and even the lifespan of the animal. One of the control mechanisms of the timing of terminal differentiation is a developmental cell intrinsic timer. Oligodendrocyte precursor cell (OPC) in a postnatal rat optic nerve carries out limited number of cell division and terminally differentiates in vitro. For example, OPC prepared from postnatal day 7 (P7) rat optic nerve divides up to 8 times. For the determination of the correct differentiation timing, two soluble factors, platelet derived growth factor (PDGF) and thyroid hormone (TH), are needed. PDGF is the major mitogen of OPC. The depletion of PDGF expires cell cycle and causes premature differentiation. And the depletion of thyroid hormone makes OPC to divide indefinitely. The sensitivity to TH administration of OPC is changed as culture day dependent manner. In early days in culture, OPC is not affected by TH administration, but in later days, OPC responds to TH immediately and starts differentiation. So we thought that the molecule which contributed the change of sensitivity to TH might be the main machinery of the cell intrinsic timer of OPC development. To find out candidates of such molecule, we tried to see the change of the gene expression profile during the OPC culture by using Affymetrix GeneChip Rat Genome 230 2.0 Array System, and found 212 of up-regulated genes.
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Research Products
(50 results)
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[Journal Article] Rb is critical in a mammalian tissue stem cell population2007
Author(s)
Wenzel PL, Wu L, de Bruin A, Chong JL, Chen WY, Dureska G, Sites E, Pan T, Sharma A, Huang K, Ridgway R, Mosaliganti K, Sharp R, Machiraju R, Saltz J, Yamamoto H, Cross JC, Robinson ML, Leone G.
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Journal Title
Genes & Development 21
Pages: 85-97
Description
「研究成果報告書概要(和文)」より
Peer Reviewed
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[Journal Article] The linoleic acid derivative DCP-LA selectively activates PKC-epsilon, possibly binding to the phosphatidylserine binding site.2006
Author(s)
Kanno T, Yamamoto H, Yaguchi T, Hi R, Mukasa T, Fujikawa H, Nagata T, Yamamoto S, Tanaka A, Nishizaki T.
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Journal Title
Journal of Lipid Research 47
Pages: 1146-1156
Description
「研究成果報告書概要(和文)」より
Peer Reviewed
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[Journal Article] Beneficial effect of intracellular free high-mannose oligosaccharides on cryopreservation of mammalian cells and proteins.2006
Author(s)
Watanabe K, Yaguchi T, Yang D, Kanno T, Nagai K, Yamamoto S, Fujikawa H, Yamamoto H, Nagata T, Tashiro C, Nishizaki T
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Journal Title
Cryobiology 53
Pages: 330-335
Description
「研究成果報告書概要(和文)」より
Peer Reviewed
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[Journal Article] A novel marker for Purkinje cells, KIAA0864 protein.An analysis based on amonoclonal antibody HFB-16 in developing human cerebellum2005
Author(s)
Yasuhiro Nakamura, Munehiko Yamamoto, Eriko Oda, Yonehiro Kanemura, Eri Kodama, Atsuyo Yamamoto, Hideyuki Yamamoto, Kenji Miyado, Hirotaka James Okano, Ryouji Fukagawa, Koichi Higaki, Mami Yamasaki and Hideyuki Okano.
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Journal Title
Journal of Histochemistry and Cytochemistry 53
Pages: 423-430
Description
「研究成果報告書概要(和文)」より
Peer Reviewed
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[Journal Article] Functional expression of ABCG2 transporter in human neural stem/progenitor cells2005
Author(s)
Islam, M.O., Kanemura, Y., Tajiria, J., Mori, H., Kobayashi, S., Hara, M., Yamazaki, M., Okano, H., Miyake, J
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Journal Title
Neuroscience Research 52
Pages: 75-82
Description
「研究成果報告書概要(和文)」より
Peer Reviewed
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[Journal Article] Characterization of ABC transporter ABCB1 expressed in human neural stem/progenitor cells2005
Author(s)
Islam, M.O., Kanemura, Y., Tajiria, J., Mori, H., Kobayashi, S., Shofuda, T., Miyake, J., Hara, M., Yamazaki, M., Okano, H
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Journal Title
FEBS Letters 579
Pages: 3473-3480
Description
「研究成果報告書概要(和文)」より
Peer Reviewed
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