2006 Fiscal Year Final Research Report Summary
Comparative genomics of gene expression mechanisms of parasitic protozoa using full-length cDNA
| Project/Area Number |
16310132
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
基礎ゲノム科学
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| Research Institution | The University of Tokyo |
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Principal Investigator |
WATANABE Junichi The University of Tokyo, Institute of Medical Science, Basic Medicine, Assistant Professor (20201189)
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| Co-Investigator(Kenkyū-buntansha) |
SUZUKI Yutaka The University of Tokyo, Graduate School of Frontier Sciences, Medical Genomics, Associate Professor (40323646)
YAMASHITA Riu The University of Tokyo, Institute of Medical Science, Human Genome Center, Assistant Professor (10401259)
SUGIMOTO Chihiro Hokkaido University, Center for Zoonosis Control, Professor (90231373)
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| Project Period (FY) |
2004 – 2006
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| Keywords | parasitic protozoa / malaria / toxoplasma / comparative genomics / gene expression / expression mechanism |
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| Research Abstract |
Parasitic protozoa are the simplest eukaryotes of which genomes have been sequenced because of their medical importance. As the attempt to study gene expression mechanism of eukaryotes, we have produced full-length cDNA libraries using the oligo-capping method which we have originated from various parasites including tertian malaria parasite, Plasmodium vivax, murine malaria parasite, P. berghei, Toxoplasma gondii, Cryptosporidium parvum, Theileria parva, T. orientalis, and Eimeria tenella, and determined 5'-end-one-pass sequences from the random clones. These sequences have been mapped onto the respective genome sequences and presented in the genome browsers. For comparative genomic analysis, a full-length cDNA library has been produced from flatworm, Echinococcus multilocularis. The results have been compiled as a database, Full-Malaria and published in internet at http : //fullmal.ims.u-tokyo.ac.jp. Furthermore, comparative genomics database, Comparasite, (http : //comparasite.hgc.ip) has been produced. These provide basic information for comparative genome analysis to elucidate the exact mechanisms of gene expression. In addition, using the original method to screen novel malaria DNA vaccine candidates, promising murine malaria vaccine candidate clones have been selected. This method should be applicable to other eukaryotic pathogens.
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