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2006 Fiscal Year Final Research Report Summary

New SEREX Antigens, Molecular Target to Diagnosis and Treatment for Esophageal Cancer

Research Project

Project/Area Number 16390372
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Digestive surgery
Research InstitutionChiba University

Principal Investigator

OCHIAI Takenori  Chiba University, GRADUATE SCHOOL OF MEDICINE, PROFESSOR, 大学院・医学研究院, 教授 (80114255)

Co-Investigator(Kenkyū-buntansha) SHIMADA Hideaki  Chiba University, GRADUATE SCHOOL OF MEDICINE, ASSISTANT PROFESSO, 大学院・医学研究院, 講師 (20292691)
HIWASA Takaki  Chiba University, GRADUATE SCHOOL OF MEDICINE, ASSOCIATE PROFESSOR, 大学院・医学研究院, 助教授 (30260251)
TOMONAGA Takashi  Chiba University, GRADUATE SCHOOL OF MEDICINE, ASSOCIATE PROFESSOR, 大学院・医学研究院, 助教授 (80227644)
Project Period (FY) 2004 – 2006
KeywordsEsophageal cancer / Squamous cell carcinoma / SEREX / tumor marker / TROP2 / GLUT-1 / SLC2A1 / Myomegalin / TRIM21
Research Abstract

In order to identify new serum markers of esophageal squamous cell carcinoma (SCC), we performed serological identification of antigens by recombinant cDNA expression cloning (SEREX). E.coli was infected with λZAPII phage cDNA library prepared from mRNA of an esophageal cancer cell line (T.Tn), and IPTG-induced cDNA products were screened for interaction with antibodies in allogeneic sera of patients with esophageal SCC. We identified TROP-2, GLUT-1/SLC2A1, TRIM21, myomegalin, as new SEREX antigens for esophageal SCC. Western blotting analysis revealed that serum anti-antibodies for these SEREX antigens were present in 20 to 47% of patients but not in healthy controls. Although the presence of serum SEREX-Abs were not related to any of clinicopathological features of the patients concerned, multivariate analysis indicated that presence of s-myomegalin-Abs was significantly associated with favorable prognosis. Moreover, s-TROP-2-Abs was associated with tumor size. Because there serum antibodies against SEREX antigens were not associated with other conventional tumor markers, there serum markers were useful as a new tumor markers for esophageal SCC.

  • Research Products

    (10 results)

All 2007 2006

All Journal Article (10 results)

  • [Journal Article] Serum anti-myomegalin antibodies in patients with esophageal squamous cell carcinoma2007

    • Author(s)
      Shimada H., Kuboshima M., Shiratori T., Nabeya Y., Takeuchi A., Takagi H., Nomura, F., Takiguchi, M., Ochiai, T., Hiwasa, T
    • Journal Title

      International Journal of Oncology 30

      Pages: 97-103

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] TSerum anti-myomegalin antibodies in patients with esophageal squamous c ell carcinoma2007

    • Author(s)
      Shimada H., Kuboshima M., Shiratori T., Nabeya Y., Takeuchi A., Takagi H., Nomura, F., Takiguchi, M., Ochiai, T.Hiwasa
    • Journal Title

      International Journal of Oncology 30

      Pages: 97-103

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Serological identification of antigens by recombinant cDNA expression cloning (SEREX) using antibodies from patients with esophageal squamous cell carcinoma2006

    • Author(s)
      Hiwasa, T., Shimada, H., Ochiai, T., Takiguchi
    • Journal Title

      Moleculomics and Thereafte

      Pages: 99-117

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Presence of serum tripartite motif-containing 21 antibodies in patients with esophageal squamous cell carcinoma2006

    • Author(s)
      Kuboshima, M., Shimada, H., Liu, T.L., Nomura, F., Takiguchi, M., Hiwasa, T., Ochiai, T
    • Journal Title

      Cancer Science 97

      Pages: 380-386

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Enhancement of chemosensitivity toward peplomycin by calpastatin-stabilized NF-kappaB p65 in esophageal carcinoma cells : possible involvement of Fas/Fas-L synergism.2006

    • Author(s)
      Liu, T.L., Shimada, H., Ochiai, T., Shiratori, T., Lin, S.E., Kitagawa, M., Harigaya, K., Maki, M., Oka, M., Abe, T., Takiguchi, M., Hiwasa, T.
    • Journal Title

      Apoptosis 11

      Pages: 1025-1037

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] An essential role of alternative splicing ofc-myc suppressor FUSE-binding protein-interacting repressor in carcinogenesis.2006

    • Author(s)
      Matsushita, K., Tomonaga, T., Shimada, H., Shioya, A., Higashi, M., Matsubara, H., Harigaya, K., Nomura, F., Libutti, D., Levens, D., Ochiai, T
    • Journal Title

      Cancer Res 66

      Pages: 1409-1417

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Proteomic analysis of primary esophageal squamous cell carcinoma reveals downregulation of a cell adhesion protein, periplakin.2006

    • Author(s)
      Nishimori, T., Tomonaga, T., Matsushita, K., Oh-Ishi, M., Kodera, Y., Maeda, T., Nomura, F., Matsubara, H., Shimada, H., Ochiai
    • Journal Title

      Proteomics 6

      Pages: 1011-1018

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Enhancement of chemosensitivity toward peplomycin by calpastatin-stabilized NF-kappaB p65 in esophageal carcinoma cells : possible involvement of Fas/Fas-L synergism.2006

    • Author(s)
      Liu, T.L., Shimada, H., Ochiai, T., Shiratori, T., Lin, S.E., Kitagawa, M., Harigaya, K., Maid, M., Oka, M., Abe, T., Takiguchi, M., Hiwasa, T.
    • Journal Title

      Apoptosis 11

      Pages: 1025-1037

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] An essential role of alternative splicing ofc-myc suppressor FUSE-binding protein-interacting repressor in carcinogenesis.2006

    • Author(s)
      Matsushita, K., Tomonaga, T., Shimada, H., Shioya, A., Higashi, M., Matsubara, H., Harigaya, K., Nomura, F., Libutti, D., Levens, D.Ochiai, T
    • Journal Title

      Cancer Res 66

      Pages: 1409-1417

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Proteomic analysis of primary esophageal squamous cell carcino ma revealsdownregulation of a cell adhesion protein, periplakin.2006

    • Author(s)
      Nishimori, T., Tomonaga, T., Matsushita, K., Oh-Ishi, M., Kodera, Y., Maeda, T., Nomura, F., Matsubara, H., Shimada, H., Ochiai
    • Journal Title

      Proteomics 6

      Pages: 1011-1048

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 2008-05-27  

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