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2006 Fiscal Year Final Research Report Summary

A basic study of gene and anti-angiogenic therapy for malignant brain tumor using bone marrow stromal cell and its potential of cell-fusion

Research Project

Project/Area Number 16390401
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Cerebral neurosurgery
Research InstitutionHokkaido University

Principal Investigator

IWASAKI Yoshinobu  Hokkaido University, Graduate School of Med., Professor, 大学院医学研究科, 教授 (00113522)

Co-Investigator(Kenkyū-buntansha) KOBAYASHI Hiroyuki  Hokkaido University, Hokkaido Univ. Hospital, Post-Doctoral Staff, 北海道大学病院, 医員 (70374478)
KURODA Satoshi  Hokkaido University, Hokkaido Univ. Hospital, Associtate Professor, 北海道大学病院, 講師 (10301904)
SHICHINOHE Hideo  Hokkaido University, Hokkaido Univ. Hospital, Post-Doctoral Staff, 北海道大学病院, 医員 (80374479)
YANO Shunsuke  Hokkaido University, Hokkaido Univ. Hospital, Associtate Professor, 北海道大学病院, 助手 (20374481)
Project Period (FY) 2004 – 2006
Keywordsbrain tumor / bone marrow stromal cell / transplantation / cell fusion / anti-angiogenesis / differentiation / biomaterial
Research Abstract

1. We have established the culture system of the bone marrow stromal cells (BMSC) obtained from the rats and mice. FACS analysis showed BMSCs expressed CD34 (-), CD45 (+/-), CD90 (+) and Sca1 (+). These data were published in Brain Res Protoc (2004).
2. The BMSCs presented active cell proliferation when it was transplanted into infarct brain. These data were published in Brain Res (2005).
3. Gene expression profiling was performed for cultured BMSCs and it was clarified that the BMSCs had potentials to alter their gene expression patterns in response to external stimuli. The data were published in Brain Res (2006).
4. Fibrin glue could be suitable biomaterial as a scaffold for the BMSCs when they were transplanted into brain cold injury model or spinal cord hemi-section model of the rats. These data has been prepared for submission to "Neurosurgery" or "Journal of Neurotrauma".
5. We examined the cell-fusion between BMSCs and different kinds of cells, and differentiation of BMSC under various conditions in vitro. Our data from immunohistochemistry suggested that the BMSCs might be fused with neuronal cells and showed neuronal differentiation in some specific circumstances. These findings are still under investigating by different methods.

  • Research Products

    (11 results)

All 2006 2005 2004

All Journal Article (11 results)

  • [Journal Article] The effects of neuronal induction on gene expression profile in bone marrow stromal cells (BMSC)-a preliminary study using microarray analysis.2006

    • Author(s)
      Yamaguchi S, Kuroda S, Kobayashi H, Iwasaki Y. et al.
    • Journal Title

      Brain Res 1087(1)

      Pages: 15-27

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Temozolomide in the treatment of recurrent malignant glioma.2006

    • Author(s)
      Kobayashi H, Sawamura Y, Ishii N, Murata J, Iwasaki Y
    • Journal Title

      No Shinkei Geka. 34(12)

      Pages: 1241-1247

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] The effects of neuronal induction on gene expression profile in bone marrow stromal cells (BMSC)-a preliminary study using microarray analysis.2006

    • Author(s)
      Yamaguchi S, Kuroda S, Kobayashi H, Shichinohe H, Yano S, Hida K, Shinpo K, Kikuchi S, Iwasaki Y
    • Journal Title

      Brain Res. 1087(1)(Epub)

      Pages: 15-27

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Temozolomide in the treatment o recurrent malignant glioma.2006

    • Author(s)
      Kobayashi H, Sawamura Y, Ishii N, Murata J Iwasaki Y
    • Journal Title

      No Shinkei Geka. 34(12)

      Pages: 1241-7

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] In vivo fluorescence tracking of bone marrow stromal cells transplanted into a pneumatic injury model of rat spinal cord.2005

    • Author(s)
      Yano S, Kuroda S, Iwasaki Y. et al.
    • Journal Title

      J Neurotrauma 22

      Pages: 907-918

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Do bone marrow stromal cells proliferate after transplantation into mice cerebral infarct?-a double labeling study.2005

    • Author(s)
      Yano S, Iwasaki Y, et al.
    • Journal Title

      Brain Res. 1065(1-2)

      Pages: 60-67

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Occipitoparietal benign osteoblastoma : should entire lesion be resected when magnetic resonance images reveal wide abnormal signal intensity in surrounding bone marrow?2005

    • Author(s)
      Narita T, Ishii N, Kobayashi H, Iwasaki Y. et al.
    • Journal Title

      Surg Neurol 64(2)

      Pages: 180-183

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] In vivo fluorescence tracking of bone marrow stromal cells transplanted into a pneumatic injury model of rat spinal cord.2005

    • Author(s)
      Yano S, Kuroda S, Lee JB, Shichinohe H, Seki T, Ikeda J, Nishimura G, Hida K, Tamura M, Iwasaki Y
    • Journal Title

      J Neurotrauma 22

      Pages: 907-918

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Do bone marrow stromal cells proliferate after transplantation into mice cerebral infarct?--a double labeling study.2005

    • Author(s)
      Yano S, Kuroda S, Shichinohe H, Hida K, Iwasaki Y
    • Journal Title

      IBrain Res. l065(1-2)

      Pages: 60-7

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Occipitoparietal benign osteoblastoma : should entire lesion be resected when magnetic resonance images reveal wide abnormal signal intensity in surrounding bone marrow?2005

    • Author(s)
      Narita T, Ishii N, Mayuzumi H, Kobayashi H, Ikeda J, Iwasaki Y
    • Journal Title

      Surg Neurol. 64(2)

      Pages: 180-3

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] A pre-clinical assessment model of rat autogeneic bone marrow stromal cell transplantation into the central nervous system.2004

    • Author(s)
      Lee JB, Kuroda S, Iwasaki Y. et al.
    • Journal Title

      Brain Res Protoc 14

      Pages: 37-44

    • Description
      「研究成果報告書概要(和文)」より

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Published: 2008-05-27  

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