2005 Fiscal Year Final Research Report Summary
The preventive mechanisms of life-style related disease by edible plant extracts and their application for the disease
| Project/Area Number |
16500515
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Eating habits, studies on eating habits
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| Research Institution | Osaka City University |
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Principal Investigator |
YUASA Isao Osaka City University, Graduate School of Human Life Science, Professor, 大学院生活科学研究科, 教授 (50094488)
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| Co-Investigator(Kenkyū-buntansha) |
KOJIMA Akiko Osaka City University, Graduate School of Human Life Science, Associate Professor, 大学院生活科学研究科, 助教授 (90295709)
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| Project Period (FY) |
2004 – 2005
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| Keywords | Edible plant extracts / Life style-related disease / Antitumor activity / Dietary fiber / Tea / Active oxygens / Cell Culture / 細胞レベル |
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| Research Abstract |
The purposes of this study are following. One is to understand the mechanisms of antitumor activity of extracts of Aloe, green tea, evening primrose and others. Another is to examined the effect of combined treatment of these extracts.
On the mechanism of antitumor activity of evening primrose extract in Ehrlich ascites tumor cells, we demonstrated that evening primrose extract (EPP) induced apoptosis and inhibited the DNA synthesis in Ehrlich ascites tumor cells (EATC) and suggested that EPE-induced inhibition of the growth of EATC are via at least two pathway differentially modulated by reactive oxygen species, notably intracellular peroxides. These are (a) the EPE-induced apoptosis pathway which is dependent on increases in hydrogen peroxide and (b) the EPE-induced inhibition of cell proliferation which is hydrogen peroxide independent. In this study EPE brought about a significant decrease in intracellular polyamine levels. Furthermore, the addition of polyamines reversed the EPE-induced decrease in cell viability and suppressed the EPE-induced increase in intracellular hydrogen peroxide. However, the addition of polyamines did not reverse EPE-induced decrease in DNA synthesis and phosphorylation of Rb protein, and EPE-induced translocation of AIF. These results suggest the involvement of polyamines in EPE-induced apoptosis pathway which is dependent on increase in hydrogen peroxide. We also examined the protective effect of green tea extract and tea polyphenols against FK-506-induced cytotoxicity in renal cells. The nephrotoxicity induced by the immunosuppressive drug FK506 limits its usefulness in widespread application.
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[Journal Article] Screening of spice and herb extracts for cytotoxic activity against Ehrlich ascites tumor cells and DPPH radical scavenging activity2004
Author(s)
Kametani, S., Matsui-Yuasa, I., Kojima-Yuasa, A., Arimura, T., Kennedy, D.O., Honzawa, M., Kikuzaki, H., Nakatani, N.
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Journal Title
ITE Letters 5
Pages: 67-75
Description
「研究成果報告書概要(欧文)」より
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[Book] 免疫と栄養2006
Author(s)
湯浅勲(分担執筆)
Total Pages
338
Publisher
幸書房
Description
「研究成果報告書概要(和文)」より
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