2005 Fiscal Year Final Research Report Summary
Rapid genetic diagnosis with the TRC (transcription-reverse transcription concerted reaction) system for cancer micrometastasis^1
| Project/Area Number |
16591310
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Digestive surgery
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| Research Institution | Osaka University |
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Principal Investigator |
FUJIWARA Yoshiyuki Osaka University, Graduate School of Medicine, Assistant, 医学系研究科, 助手 (40314330)
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| Co-Investigator(Kenkyū-buntansha) |
TAKIGUCHI Shuji Osaka University, Graduate School of Medicine, Assistant, 医学系研究科, 助手 (00301268)
MIYATA Hiroshi Osaka University, Graduate School of Medicine, Assistant, 医学系研究科, 助手 (80362713)
YASUDA Takushi Osaka University, Graduate School of Medicine, Assistant, 医学系研究科, 助手 (10324782)
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| Project Period (FY) |
2004 – 2005
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| Keywords | genetic diagnosis / micrometastasis / TRC / RNA amplification / gastric cancer / peritoneal lavage |
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| Research Abstract |
Detection of cancer micrometastases that are missed by conventional cytological and histopathological examination is required for improvement of cancer therapy. The aim of this study was to establish a rapid and practical genetic assay to detect micrometastasis in gastric cancer and to assess its clinical significance with respect to prognosis.
A novel RNA amplification system with TRC reaction (transcription-reverse transcription concerted reaction) was introduced for quantitative detection of CEA (carcinoembryonic antigen) mRNA, which is widely used as a molecular marker for cancer micrometastasis. Peritoneal lavage fluid specimens and lymph nodes collected from cancer surgery were subjected to the assay, and the clinical significance of the results in prediction of recurrence and survival was examined.
The quantification, sensitivity, and reproducibility of the assay using the TRC reaction were equal to those of quantitative RT-PCR with LightCycler^<TM>. The most important advantage of the assay was its simplicity and rapidity. Molecular diagnosis of peritoneal lavage fluid by the TRC reaction significantly correlated with depth of invasion, peritoneal metastasis, clinical stage, overall survival and peritoneal recurrence-free survival.
Molecular diagnosis of peritoneal lavage fluid with the TRC reaction could be a useful prognostic indicator for peritoneal recurrence and survival. Because the TRC reaction is more rapid and simple than RT-PCR as a format for detecting RNA sequences, it may enhance the genetic diagnosis of cancer micrometastasis and may contribute to cancer therapy.
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