2005 Fiscal Year Final Research Report Summary
Modification of the Hepatic Mitochondrial Proteome in Response to Ischemic Preconditioning Following Ischemia-Reperfusion Injury of the Rat Steatotic Liver.
| Project/Area Number |
16591367
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Digestive surgery
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| Research Institution | St.Marianna University School of Medicine |
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Principal Investigator |
NAKANO Hiroshi St.Marianna University School of Medicine, Department of Gastroenterological Surgery, Lecturer, 医学部, 講師 (10241035)
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| Co-Investigator(Kenkyū-buntansha) |
KOBAYASHI Shinichi St.Marianna University School of Medicine, Department of Pharmacology, Professor, 医学部, 教授 (20129836)
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| Project Period (FY) |
2004 – 2005
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| Keywords | Steatotic liver / Zucker rat / Ischemic preconditioning / Ischemia reperfusion / 2D-DIGE / Proteomics / Mitochoindria |
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| Research Abstract |
Background/Aim : Ischemic preconditioning (IPC) has been shown to reduce hepatic ischemia-reperfusion (IR) injury of steatotic liver, but the effect of IPC on mitochondrial proteome has not been fully demonstrated. The present study investigated how IPC modifies the mitochondrial proteome after IR injury of the rat steatotic liver.
Methods : Zucker steatotic rats were subjected to 25-min portal triad cross-clamping (IR group). In the IPC group, 10-min temporal clamping followed by 10-min reperfusion was performed before the 25-min clamping. The other rats were sham-operated as a control group. Mitochondrial proteome was analyzed 24 hours after IR using two-dimensional differential in-gel electrophoresis and mass spectrometry.
Results : Mitochondrial inner-membrane potential and glutathione were lower and serum transaminase was higher in the IPC group than in the IR group. There was a greater degree of up-regulation of the mitochondrial precursor of aldehyde dehydrogenase 2 and alpha-methylacyl-CoA racemase in the IPC group in comparison to the IR group. In contrast, 60S acid ribosomal protein P0, carbonic anhydrase 3, arginase 1, and superoxide dismutase were significantly more down-regulated in the IPC group than in the IR group.
Conclusions : A hepato-protective effect was not shown by IPC ; however, IPC caused significant up-or down-regulation of several mitochondrial proteins 24 hours after IR of steatotic liver.
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