2005 Fiscal Year Final Research Report Summary
Study on the molecular mechanism of secretory granule formation of pituitary hormone
| Project/Area Number |
16591461
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
Cerebral neurosurgery
|
|---|
| Research Institution | Teikyo University |
|---|
Principal Investigator |
MATSUNO Akira Teikyo University, School of Medicine, Professor, 医学部, 教授 (00242058)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
NAGASHIMA Tadashi Teikyo University, School of Medicine, Professor, 医学部, 教授 (70217991)
FUJIMAKI Takamitsu Teikyo University, School of Medicine, Associate Professor, 医学部, 助教授 (80251255)
NAKAGOMI Tadayoshi Teikyo University, School of Medicine, Professor, 医学部, 教授 (90198052)
TAKEKOSHI Susumu Tokai University, School of Medicine, Associate Professor, 医学部, 助教授 (70216878)
OSAMURA Yoshiyuki Tokai University, School of Medicine, Professor, 医学部, 教授 (10100992)
|
|---|
| Project Period (FY) |
2004 – 2005
|
| Keywords | Growth hormone / pituitary cell / secretory granule / GFP / chromogranin A / secretogranin 3 / carboxypeptidase E / phogrin |
|---|
| Research Abstract |
In order to investigate, in real time, the transport and secretion of pituitary hormone, we have developed an experimental pituitary cell line, GH3 cell, which has secretory granules of GH linked to EYFP. This stable GH3 cell secretes secretory granules of GH linked to EYFP upon stimulated by Ca^<2+> influx or Ca^2 release from storage. This GH3 cell will be useful for the real-time visualization of the intracellular transport and secretion of GH. Moreover, we have cotransfected rab3B linked to ECYP in this GH3 cell, and have elucidated the role of rab3B in GH secretion. Using the similar cotransfection system, we have elucidated the role of chromogranin A, secretogranin 3, carboxypeptidase E, and phogrin in the mechanism of secretory granule formation in pituitary cell.
|
