2006 Fiscal Year Final Research Report Summary
Effect of positive end-expiratory pressure on inflammatory response in oleic acid-induced lung injury and whole lung lavage-induced lung injury
| Project/Area Number |
16591528
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Anesthesiology/Resuscitation studies
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| Research Institution | Tokyo Medical and Dental University |
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Principal Investigator |
NAKAZAWA Koichi Tokyo Medical and Dental University, Department of Anesthesiology & Critical Care Medicine, Associate Professor, 大学院医歯学総合研究科, 助教授 (10207756)
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| Co-Investigator(Kenkyū-buntansha) |
YOKOYAMA Kuninori Tokyo Medical and Dental University, Department of Anesthesiology & Critcal Care Medicine, Assistant Professor, 大学院医歯学総合研究科, 助手 (00014176)
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| Project Period (FY) |
2004 – 2006
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| Keywords | ARDS / PEEP / Oleic acid induced lung injury / Lung lavage / IL-8 |
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| Research Abstract |
The present study investigated the effects of PEEP on two different lung injury models : edematous lung induced by oleic acid (OA); and atelectatic lung induced by whole lung lavage (LAV). Japanese white rabbits (n=28) were allocated into one of the two lung injury (OA or LAV) groups and each group was treated with intermittent positive pressure ventilation using zero end-expiratory pressure (ZEEP) or PEEP (1cmH2O above the lower inflection point). Therefore, animals were divided into LAV-ZEEP, LAV-PEEP, OA-ZEEP or OA-PEEP group. Blood and bronchoalveolar fluid were sampled 3 h after ventilatory treatment to analyze IL-8 levels. Main Results : PaO_2 was significantly decreased after induction of lung injury, but was significantly higher in PEEP groups compared to ZEEP groups in each lung injury. Serum IL-8 levels were elevated in both experimental models. Serum IL-8 levels were significantly lower in LAV-PEEP than in LAV-ZEEP, whereas no difference was noted between OA-PEEP and OA-ZEEP. BALF IL-8 levels were lower in LAV-PEEP than in LAV-ZEEP. PEEP above LIP attenuates elevation of IL-8 in BALF and serum in atelectatic lungs, but does not attenuate these increases in edematous lung. In conclusion, the protective effects of PEEP on injured lungs may depend on underlying lung pathology.
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