• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to project page

2006 Fiscal Year Final Research Report Summary

Analysis of steroid hormone receptor mRNA isoforms/variants in the human endometrial carcinoma.

Research Project

Project/Area Number 16591651
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Obstetrics and gynecology
Research InstitutionUniversity of Yamanashi

Principal Investigator

HIRATA Shuji  University of Yamanashi, Department of Research Interdisciplinary Graduate School of Medicine and Engineering, Associate Professor, 医学工学総合研究部, 助教授 (00228785)

Co-Investigator(Kenkyū-buntansha) HOSHI Kazuhiko  University of Yamanashi, University of Yamanashi Hospital, Professor, 医学部附属病院, 教授 (20111289)
KASAI Tuyoshi  University of Yamanashi, University of Yamanashi Hospital, Assistant Professor, 医学部附属病院, 講師 (20194699)
SHODA Tomoko  University of Yamanashi, Department of Research Interdisciplinary Graduate School of Medicine and Engineering, Research Associate, 大学院医学工学総合研究部, 助手 (50345716)
Project Period (FY) 2004 – 2006
Keywordssteroid hormone receptor / isoform / endometrial carcinoma / variant / gene expression / quantative PCR / estrogen receptor / progesterone receptor
Research Abstract

There is six isoforms of estrogen receptor alpha (ERα), beta (ERβ) and progesterone receptor (PR). Their transcriptions are controlled by the promoter distinct from wild type (multiple promoter mechanism). This study analyzed some expression of the steroid hormone receptor (SHR) isoforms in the uterine endometrium. We have established the isoform specific quantative RT-PCR assay. In addition we analyzed the SHR isoform gene expression in the normal endometirum and endometrial carcinoma. The levels of SHR isoform mRNAs are different from those of the wild type in the tissues, indicating the different multiple promoter activities in the carcinoma. However, we introduced the clone technique by somatic cell nuclear transfer because this study was difficult by the analysis of the single promoter with the conventional reporter constructs. By the cancer cell nuclear transfer to the enucleated oocytes, we established the ES cell from the embryo (ntES cell). Now, using this ntES cell, we study to elucidate gene regulation by the multiple promoter mechanism. In addition, we have studied the PHGPx isoform specific gene expression was regulated the multiple promoter system. We already studied the change of the level of the isoform specific gene expression by the SH such as estrogen. The multiple promoter system analysis with the somatic cell nuclear transfer technique may produce the novel findings in the field.

  • Research Products

    (32 results)

All 2006 2005 2004

All Journal Article (27 results) Book (5 results)

  • [Journal Article] Correlation of Abnormal Mitochondrial Distribution in Mouse Oocyte with Reduced Developmental Competence.2006

    • Author(s)
      S Nagai, T MABUCHI, S HIRATA, T SHODA, T KASAI, その他5名
    • Journal Title

      Tohoku J. Exp. Med. 210

      Pages: 137-144

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] 臨床分子内分泌学4-生殖内分泌系-VI.プロゲステロン プロゲステロンの基礎研究 プロゲステロン受容体2006

    • Author(s)
      正田 朋子, 平田 修司, 星 和彦, 加藤順三
    • Journal Title

      日本臨床 64・4

      Pages: 430-433

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] 臨床分子内分泌学4-生殖内分泌系-V.エストロゲン エストロゲンの基礎研究エストロゲン受容体2006

    • Author(s)
      正田 朋子, 平田 修司, 星 和彦, 加藤順三
    • Journal Title

      日本臨床 64・4

      Pages: 350-354

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] 特集 配偶子形成から受精まで 4.精巣機能と精子形成 -精子形成における減数分裂の特色とstemcellを応用した精子形成について-2006

    • Author(s)
      正田 朋子, 平田 修司, 星 和彦, 加藤順三
    • Journal Title

      産科と婦人科 73・6

      Pages: 719-726

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] 尿失禁のスクリーニング2006

    • Author(s)
      大森真紀子, 平田 修司, 星 和彦
    • Journal Title

      産婦人科の実際 55・11

      Pages: 1965-1972

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Sperm. PHGPx of sperm.2006

    • Author(s)
      Shoda T
    • Journal Title

      Tokyoudai shuppan

      Pages: 310-314

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] ART Manual.2006

    • Author(s)
      Shoda T
    • Journal Title

      Nagai Shoten

      Pages: 67-78

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Correlation of Abnormal Mitochondria Distribution in Mouse Oocyte with Reduced Developmental Competence.2006

    • Author(s)
      Nagai S
    • Journal Title

      Tohoku J. Exp. Med. 210

      Pages: 137-144

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Progesterone Receptor.2006

    • Author(s)
      Shoda T
    • Journal Title

      Nihon Rinsho 64(4)

      Pages: 430-433

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Estrogen Receptor.2006

    • Author(s)
      Shoda T
    • Journal Title

      Nihon Rinsho 64(4)

      Pages: 350-354

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] From Spermatogenesis to fertilization.2006

    • Author(s)
      Shoda T
    • Journal Title

      Sanka to Fujinka 73(6)

      Pages: 719-726

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Screening of urinary in continence.2006

    • Author(s)
      Oomori M
    • Journal Title

      Sanfujinka no jissai 55(11)

      Pages: 1965-1972

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Chromatoid bodies : aggresome-like characteristics and degradation sites for organelles of spermiogenic cells.2005

    • Author(s)
      Haraguchi CM, Mabuchi T, Hirata S, Shoda T, Hoshi K, Akasaki K, Yokota S
    • Journal Title

      J Histochem Cytochem. 53(4)

      Pages: 455-465

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Novel human alpha-fetoprotein mRNA isoform lacking exon 1 identified in ovarian yolk sac tumor.2005

    • Author(s)
      Fukasawa H, Iwamoto H, Hirata S, Shoda T, Yokota S, Nishi S, Hoshi K
    • Journal Title

      J Soc Gynecol Investig. 12(6)

      Pages: 456-462

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] ヒト女性ステロイドホルモン受容体のisoformおよびvariant mRNAについて-エストロゲン受容体a(FRa)、エストロゲン受容体b(ERb)、プロゲステロン受容体(PR)-2005

    • Author(s)
      正田朋子, 平田修司, 星 和彦
    • Journal Title

      日本生殖内分泌学会雑誌 10

      Pages: 9-14

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Novel human alpha-fetoprotein mRNA isoform lacking exon 1 identified in ovarian yolk sac tumor.2005

    • Author(s)
      Fukasawa H
    • Journal Title

      J Soc Gynecol Investig. 12(6)

      Pages: 456-462

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] The isoform/variants mRNA of the steroid hormone receptor (ERα, ERβ and PR).2005

    • Author(s)
      Shoda T
    • Journal Title

      Japanese J of Productive Endocrinology 10

      Pages: 9-14

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Chromatoid bodies : Aggresome-like characteristics and degradation sites for organelles of spermiogenic cells.2005

    • Author(s)
      Haraguchi CM
    • Journal Title

      J Histochem Cytochem 53(4)

      Pages: 455-465

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Text for the embolist. IV. Basic and Rinsho of sperm.2005

    • Author(s)
      Shoda T
    • Journal Title

      Kindai 87-94

      Pages: 87-94

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Progress of IVF.2005

    • Author(s)
      Shoda T
    • Journal Title

      Medical view 260-263

      Pages: 260-263

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Ubiquitin signals in the developing acrosome during spermatogenesis of rat testis : AnImmunoelectron microscopic study.2004

    • Author(s)
      Celina M.Haraguchi, Tadashi MABUCHI, Shuji HIRATA, Tomoko SHODA, Kazuhiko HOSHI, Sadaki YOKOTA
    • Journal Title

      JOURNAL OF HISTOCHEMISTRY & CYTOCHEMISTRY 52(11)

      Pages: 1393-1403

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Effect of repeated administration of clomiphene citrate at two different times on the endometrium in patients undergoing intrauterine insemination.2004

    • Author(s)
      Mamiko WADA, Tsuyoshi KASAI, Seiichiro NAGAI, Michiko FUJIE, Maki MIYAKE, Kohta Suzuki, Mariko SUZUKI, Shuji HIRATA, Kazuhiko HOSHI
    • Journal Title

      Reproductive Medicine and Biology 3(3)

      Pages: 153-157

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Cytoplasmic transfer in the mouse in conjunction with intracytoplasmic sperm injection.2004

    • Author(s)
      Seiichiro NAGAI, Shuji HIRATA, Tuyoshi KASAI, Kazuhiko HOSHI, Ryuzo YANAGIMACHI
    • Journal Title

      Reproductive BioMedicine Online 8(1)

      Pages: 75-80

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Effect of repeated administration of clomiphene citrate at two different times on the endometrium in patients undergoing intrauterine insemination.2004

    • Author(s)
      Wada M
    • Journal Title

      Reprod Med Biol 3 (3)

      Pages: 153-157

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Ubiquitin signals in the developing acrosome during spermatogenesis of rat testis : An Immunoelectron microscopic study.2004

    • Author(s)
      Haraguchi CM
    • Journal Title

      J Histochem Cytochem 52 (11)

      Pages: 1393-1403

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Cytoplasm transfer in the mouse in conjunction with intracytoplasmic sperm injection.2004

    • Author(s)
      Nagai S
    • Journal Title

      Reproductive Bio Medicine Online 8(1)

      Pages: 75-80

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] The Mechanism of spermatogenesis.2004

    • Author(s)
      Shoda T
    • Journal Title

      Hormone Frontier in GYnecology 11(3)

      Pages: 235-242

    • Description
      「研究成果報告書概要(欧文)」より
  • [Book] 図説 ARTマニュアル改訂第二版1.受精の基礎知識 第3章 配偶子と受精1.受精の基礎知識2006

    • Author(s)
      正田朋子, 平田 修司, 星 和彦
    • Total Pages
      538 (67-78)
    • Publisher
      永井書店
    • Description
      「研究成果報告書概要(和文)」より
  • [Book] 新編 精子学 精子とPHGPx2006

    • Author(s)
      正田朋子, 平田 修司, 横田 貞記, 馬淵 正, 星 和彦
    • Total Pages
      490 (310-314)
    • Publisher
      東京大学出版会
    • Description
      「研究成果報告書概要(和文)」より
  • [Book] 「生命の誕生にむけて-生殖補助医療胚培養士講習会テキスト-」IV.精子の基礎と臨床1.精子の形成と成熟2005

    • Author(s)
      正田朋子, 平田修司, 星 和彦
    • Total Pages
      87-94
    • Publisher
      近代出版
    • Description
      「研究成果報告書概要(和文)」より
  • [Book] 改訂第4版進化していく体外受精progress「受精障害の解明」2005

    • Author(s)
      正田朋子, 平田修司, 星 和彦
    • Total Pages
      260-263
    • Publisher
      Medical View
    • Description
      「研究成果報告書概要(和文)」より
  • [Book] Hormone Frontier in Gynecology11(3) : 精子成熟機構2004

    • Author(s)
      正田朋子
    • Total Pages
      235-242
    • Publisher
      株式会社 メディカルレビュー
    • Description
      「研究成果報告書概要(和文)」より

URL: 

Published: 2008-05-27  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi