Establishment of Neuroglycobiol ogy (Glycobiological Approach for Neuroscience)

2008 Fiscal Year Final Research Report

• Project/Area Number: 16GS0313
• Research Category: Grant-in-Aid for Creative Scientific Research
• Allocation Type: Single-year Grants
• Research Institution: Kyoto University
• Principal Investigator: OKA Shogo Kyoto University, 医学研究科, 教授 (60233300)
• Co-Investigator(Kenkyū-buntansha): MA BRUCE・Yong 立命館大学, COE推進機構, 准教授 (00378788) ; KOZUTSUMI Yasunori 京都大学, 生命科学研究科, 教授 (70205425) ; KINOSITA Masato 京都大学, 農学研究科, 助教 (60263125) ; KAWASAKI Nana 国立薬品食品衛生研究所, 生物薬品部, 室長 (20186167) ; SEKI Tatsunori 東北大学, 医学研究科, 准教授 (20175417)
• Co-Investigator(Renkei-kenkyūsha): ITOH Nobuyuki 京都大学, 薬学研究科, 教授 (10110610)
• Project Period (FY): 2004 – 2008
• Keywords: 糖鎖生物学 / 神経発生 / 神経回路形成

Research Abstract

生物はタンパク質だけでなく糖鎖にもその生体情報を書き込み、同一タンパク質に多様な機能を付加していると考えられる。神経発生、神経回路形成は細胞の分化、移動、軸索の伸長、標的細胞の認識、シナプス形成など多くの過程を経て行われる。これらの過程において特徴的な発現を示す糖鎖について研究を行い、糖鎖が神経発生、神経可塑性において重要な役割を担っていること示す知見を得た。

Research Products

• [Journal Article] Regulated expression of HNK-1 carbohydrate is essential for medaka (Oryzias latipes) embryogenesis (2009)
  • Author(s): D. Anzai, Y. Tonoyama, A. Ikeda, T. Kawasaki and S. Oka
  • Journal Title: Glycobiology (in press)
  • Peer Reviewed
• [Journal Article] Essential role of beta 1,4-galactosyltransferase 2 during medaka (Oryzias latipes) gastrulation (2009)
  • Author(s): Y. Tonoyama, D. Anzai, A. Ikeda, S. Kakuda, M. Kinoshita, T. Kawasaki, S. Oka
  • Journal Title: Mech. Dev (in press)
  • Peer Reviewed
• [Journal Article] Learning/memory impairment and reduced expression of the HNK-1 carbohydrate in beta 4-galactosyltransferase-II-deficient mice (2009)
  • Author(s): T. Yoshihara, K. Sugihara, Y. Kizuka, S. Oka and M. Asano
  • Journal Title: J. Biol. Chem 284 (18) Pages: 12550-12561
  • Peer Reviewed
• [Journal Article] Distinct transport and intracellular activities of two GlcAT-P isoforms (2009)
  • Author(s): Y. Kizuka, Y. Tonoyama and S. Oka
  • Journal Title: J. Biol. Chem 284(14) Pages: 9247-9256
  • Peer Reviewed
• [Journal Article] Highly Fucosylated N-Glycan Ligands for Mannan-binding Protein Expressed Specifically on CD26 (DPPVI) Isolated from a Human Colorectal Carcinoma Cell Line (2009)
  • Author(s): N. Kawasaki, CW. Lin CW, R. Inoue, KH. Khoo, N. Kawasaki, BY. Ma, S. Oka, M. Ishiguro, T. Sawada, H. Ishida, T. Hashimoto and T. Kawasaki
  • Journal Title: SW1116. Glycobiology 19(4) Pages: 437-450
  • Peer Reviewed
• [Journal Article] Expression of cardiac myosin light chain 2 (mcmlc2) during embryonic heart development in medaka fish, Oryzias latipes, and phylogenetic relationship with other myosin light chains (2009)
  • Author(s): E. Shimada, M. Kinoshita and K. Murata
  • Journal Title: Develop. Growth Differ 51 Pages: 1-16
  • Peer Reviewed
• [Journal Article] Development and application of high performance liquid chromatography map of glucuronyl N-glycans (2008)
  • Author(s): H. Yagi, K. Yamada, E. Ohno, M. Utsumi, Y. Yamaguchi, E. Kurimoto, N. Takahashi N, S. Oka, T. Kawasaki and K. Kato
  • Journal Title: Open Glycoscience 1 Pages: 8-18
  • Peer Reviewed
• [Journal Article] Expression and function of the HNK-1 carbohydrate (2008)
  • Author(s): I. Morita, Y. Kizuka, S. Kakuda and S. Oka
  • Journal Title: J. Biochem 43 Pages: 719-724
  • Peer Reviewed
• [Journal Article] Laminin-1 is a novel carrier glycoprotein for the non-sulfated HNK-1 epitope in mouse kidney (2008)
  • Author(s): Y. Kizuka, K. Kobayashi, S. Kakuda, Y. Nakajima, S. Itoh, N. Kawasaki and S. Oka
  • Journal Title: Glycobiology 18 Pages: 331-338
  • Peer Reviewed
• [Journal Article] Polysialic acid regulates the clustering, migration, and neuronal differentiation of progenitor cells in the adult hippocampus (2008)
  • Author(s): A. Burgess, SR. Wainwright, LS. Shihabuddin, U. Rutishauser, T. Seki and I.. Aubert
  • Journal Title: Develop Neurobiol 68 Pages: 1580-1590
  • Peer Reviewed
• [Journal Article] Glycosylation analysis of IgLON family proteins in rat brain by liquid chromatography/multiple-stage mass spectrometry (2008)
  • Author(s): S. Itoh, A. Hachisuka, N. Kawasaki, N. Hashii, R. Teshima, T. Hayakawa, T. Kawanishi and T. Yamaguchi
  • Journal Title: Biochemistry 47 Pages: 10132-10154
  • Peer Reviewed
• [Journal Article] Distributions of glucuronyltransferases, GlcAT-P and GlcAT-S, and their target substrate, the HNK-1 carbohydrate epitope in the adult mouse brain with or without a targeted deletion of the GlcAT-P gene (2007)
  • Author(s): M. Inoue, K. Kato, H. Matsuhashi, Y. Kizuka, T. Kawasaki and S. Oka
  • Journal Title: Brain Res 1179 Pages: 1-15
  • Peer Reviewed
• [Journal Article] Isolation, cloning, and characterization of a novel phosphomannan-binding lectin from porcine serum (2007)
  • Author(s): BY. Ma, N. Nakamura, V. Dlabac, H. Naito, S. Yamaguchi, M. Ishikawa, M. Nonaka, M. Ishiguro, N. Kawasaki, S. Oka and T. Kawasaki
  • Journal Title: J. Biol. Chem 282 (17) Pages: 12963-75
  • Peer Reviewed
• [Journal Article] LPS suppresses expression of asialoglycoprotein-binding protein through TLR4 in thioglycolate-elicited peritoneal macrophages (2007)
  • Author(s): BY. Ma, M. Kaihama, M. Nonaka, S. Oka, N. Kawasaki and T. Kawasaki
  • Journal Title: Glycoconj. J 24 Pages: 243-249
  • Peer Reviewed
• [Journal Article] Clustering, migration and neurite formation of neural precursor cells in the adult rat hippocampus (2007)
  • Author(s): T. Seki, T. Namba, H. Mochizuki and M. Onodera
  • Journal Title: J Comp Neurol 502 Pages: 275-290
  • Peer Reviewed
• [Journal Article] Correlation Index-Based Responsible-Enzyme Gene Screening (CIRES), a Novel DNA Microarray-Based Method for Enzyme Gene Involved in Glycan Biosynthesis (2007)
  • Author(s): H. Yamamoto, H. Takematsu, R. Fujinawa, Y. Naito, Y. Okuno, G. Tsujimoto, A. Suzuki and Y. Kozutsumi
  • Journal Title: PLoS ONE 2 Pages: e1232
  • Peer Reviewed
• [Journal Article] Germinal center marker GL7 probes activation-dependent repression of N-glycolylneuraminic acid, a sialic acid species involved in the negative modulation of B-cell activation (2007)
  • Author(s): Y. Naito, H. Takematsu, S. Koyama, S. Miyake, H. Yamamoto, R. Fujinawa, M. Sugai, Y. Okuno, G. Tsujimoto, T. Yamaji, Y. Hashimoto, S. Itohara, T. Kawasaki, A. Suzuki and Y. Kozutsumi
  • Journal Title: Mol. Cell Biol 27 Pages: 3008-3022
  • Peer Reviewed
• [Journal Article] Brorin a novel secreted BMP antagonist, promotes neurogenesis in mouse neural precursor cells (2007)
  • Author(s): N. Koike, Y. Kassai, Y. Kouta, H. Miwa, M. Konishi and N. Itoh
  • Journal Title: J. Biol. Chem 282 Pages: 15843-15850
  • Peer Reviewed
• [Journal Article] Crystal structure of GlcAT-S, a human glucuronyltransferase, involved in the biosynthesis of the HNK-1 carbohydrate epitope (2006)
  • Author(s): T. Shiba, S. Kakuda, M. Ishiguro, I. Morita, S. Oka, T. Kawasaki, S. Wakatsuki and R. Kato
  • Journal Title: Proteins 65 (2) Pages: 499-508
  • Peer Reviewed
• [Journal Article] Physical and functional association of glucuronyltransferases and sulfotransferase glucuronyltransferases and sulfotransferase involved in HNK-1 biosynthesis (2006)
  • Author(s): Y. Kizuka, T. Matsui, H. Takematsu, Y. Kozutsumi, T. Kawasaki and S. Oka
  • Journal Title: J. Biol. Chem. 281(19) Pages: 13644-13651
  • Peer Reviewed
• [Journal Article] N-linked oligosaccharide analysis by liquid chromatography with graphitized carbon column/ liner ion trap-Fourier transform ion cyclotron resonance mass spectrometry in positive and negative ion modes (2006)
  • Author(s): S. Itoh, N. Kawasaki, N. Hashii, A. Harazono, Y. Matsuishi, T. Kawanishi and T. Hayakawa
  • Journal Title: J. Chromatogr. A 1103 Pages: 296-306
  • Peer Reviewed
• [Journal Article] Mannan-binding protein blocks the activation of metalloproteases meprin alpha and beta (2005)
  • Author(s): M. Hirano, BY. Ma, N. Kawasaki, K. Okimura, M. Baba, T. Nakagawa, K. Miwa, N. Kawasaki, S. Oka and T. Kawasaki
  • Journal Title: J. Immunology 175 (5) Pages: 3177-3185
  • Peer Reviewed
• [Journal Article] Non-sulfated form of the HNK-1 carbohydrate is expressed in mouse kidney (2005)
  • Author(s): H. Tagawa, Y. Kizuka, T. Ikeda, S. Itoh, N. Kawasaki, H. Kurihara, M. L. Onozato, A. Tojo, T. Sakai, T. Kawasaki and S. Oka
  • Journal Title: J. Biol. Chem 280 (25) Pages: 23876-23883
  • Peer Reviewed
• [Journal Article] Characterization of oligosaccharide ligands expressed on SW1116 cells recognized by Mannan-binding protein a highly fucosylated polylactosamine type N-glycan (2005)
  • Author(s): M.Terada, K.H. Khoo, R. Inoue, C.I. Chen, K. Yamada, H. Sakaguchi, N. Kadowaki, BY. Ma, S. Oka, T. Kawasaki and N. Kawasaki
  • Journal Title: J. Biol. Chem. 280 (12) Pages: 10897-10913
  • Peer Reviewed
• [Journal Article] Different acceptor specificities of two glucuronyltransferases involved in the biosynthesis of HNK-1 carbohydrate (2005)
  • Author(s): S. Kakuda, Y. Sato, Y. Tonoyama, S. Oka and T. Kawasaki
  • Journal Title: Glycobiology 15 (2) Pages: 203-210
  • Peer Reviewed
• [Journal Article] Fgf19 regulated by Hh signaling is required for zebrafish forebrain development (2005)
  • Author(s): A. Miyake, Y. Nakayama, M. Konishi and N. Itoh
  • Journal Title: Dev. Biol 228 Pages: 259-275
  • Peer Reviewed
• [Journal Article] Superoxide production from human polymorphonuclear leukocytes by human mannan-binding protein (MBP) (2004)
  • Author(s): K. Uemura, H. Yamamoto, T. Nakagawa, K. Nakamura, N. Kawasaki, S. Oka, B.Y. Ma and T. Kawasaki
  • Journal Title: Glycoconj J 21 Pages: 79-84
  • Peer Reviewed
• [Journal Article] Structural basis for acceptor substrate recognition of a human glucuronyltransferase, GlcAT-P, an enzyme critical in the biosynthesis of the carbohydrate epitope HNK-1 (2004)
  • Author(s): S. Kakuda, T. Shiba, M. Ishiguro, H. Tagawa, S. Oka, Y. Kajiwara, T. Kawasaki, S. Wakatsuki and R. Kato
  • Journal Title: J Biol Chem 279 Pages: 22693-22703
  • Peer Reviewed
• [Journal Article] Purification and characterization of two recombinant human glucuronyltransferases involved in the biosynthesis of HNK-1 carbohydrate in Escherichia coli (2004)
  • Author(s): S. Kakuda, S. Oka, T. Kawasaki
  • Journal Title: Protein Expr Purif 35 Pages: 111-119
  • Peer Reviewed
• [Presentation] Two GlcAT-P isoforms are distinctly transported in cell. Clinical and Translational Research on Cancer (2009)
  • Author(s): Y. Kizuka, Y. Tonoyama, S. Oka
  • Organizer: Glycomics Applications
  • Place of Presentation: 伊勢
  • Year and Date: 2009-03-26
• [Presentation] The functional analysis of HNK-1 carbohydrate in medaka embryogenesis Joint meeting of the Socie'te' France de Biologie du De'velopment (2008)
  • Author(s): D. Anzai, Y. Tonoyama, E. Tane, S. Oka
  • Organizer: Japanese Society of Developmental Biologists
  • Place of Presentation: フランスGiens
  • Year and Date: 20080913-17
• [Presentation] GlcAT-P遺伝子欠損マウスにおける残存HNK-1糖鎖発現に関する研究 (2008)
  • Author(s): 山下彩貴、木塚康彦、角田品子、岡昌吾
  • Organizer: 糖鎖発現に関する研究第128年回日本薬学会
  • Place of Presentation: 横浜
  • Year and Date: 20080326-28
• [Presentation] 発生過程に重要な糖鎖の機能解 (2008)
  • Author(s): 岡昌吾
  • Organizer: 第128年回日本薬学会
  • Place of Presentation: 横浜
  • Year and Date: 20080326-28
• [Presentation] 糖転移酵素GlcAT-Pの細胞質領域による分泌制御と活性調節 (2008)
  • Author(s): 木塚康彦、竹内祐介、森田一平、角田品子、岡昌吾
  • Organizer: 第28回日本糖質学会
  • Place of Presentation: つくば
  • Year and Date: 2008-08-20
• [Presentation] スパイン形成におけるHNK-1糖鎖の役割 (2008)
  • Author(s): 岡昌吾
  • Organizer: 第31 回日本神経科学学会
  • Place of Presentation: 東京
  • Year and Date: 2008-07-10
• [Presentation] Beta1,4-galactosyltransferase 2 is required for convergence and extension movements during medaka (Oryzias latipes) gastrulation (2008)
  • Author(s): 殿山泰弘、安西大輔、池田篤史、角田品子、木下政人、川崎敏祐、岡昌吾
  • Organizer: 第41回日本発生生物学会(国際発生生物学会と合同)
  • Place of Presentation: 徳島
  • Year and Date: 2008-05-28
• [Presentation] 神経機能に重要なHNK-1糖鎖BMB2007 (2007)
  • Author(s): 岡昌吾
  • Organizer: 第30回日本分子生物学会年会、第80回日本生化学会大会合同大会
  • Place of Presentation: 横浜
  • Year and Date: 20071211-15
• [Presentation] 神経可塑性とHNK-1糖鎖 (2007)
  • Author(s): 岡昌吾
  • Organizer: 第5回糖鎖科学コンソーシアムシンポジウム
  • Place of Presentation: 東京
  • Year and Date: 20071126-27
• [Presentation] Essential role of beta1,4-galactosyltransferase 2 in convergence and extension movements during medaka (Oryzias latipes) embryogenesi (2007)
  • Author(s): Y. Tonoyama, D. Anzai, A. Ikeda, S. Kakuda, M. Kinoshita, T. Kawasaki, S. Oka
  • Organizer: HUPO 6th annual world congress
  • Place of Presentation: Seoul, Korea
  • Year and Date: 20071006-10
• [Presentation] Characterization of a unique HNK-1 (Human Natural Killer-1) carbohydrate on phosphacan in mouse brain. Glyco19 (2007)
  • Author(s): Y. Kizuka, N. Kawasaki, N. Maeda, T. Kawasaki and S. Oka
  • Organizer: XIX International Symposium on Glycoconjugates
  • Place of Presentation: Cairns, Australia
  • Year and Date: 20070715-20
• [Presentation] 神経可塑性におけるHNK-1糖鎖の役割 (2007)
  • Author(s): 岡昌吾
  • Organizer: 日本薬学会第127年会
  • Place of Presentation: 富山
  • Year and Date: 20070300
• [Presentation] HNK-1糖鎖の機能 (2006)
  • Author(s): 岡昌吾
  • Organizer: 第4回糖鎖科学コンソーシアムシンポジウム
  • Place of Presentation: 東京
  • Year and Date: 20061023-24
• [Presentation] HNK-1糖鎖生合成酵素GlcAT-S遺伝子欠損マウスの作成とその生化学的解釈 (2006)
  • Author(s): 小林恭子, 陣内俊和, 角田品子, 木塚康彦, 高橋智, 川崎敏祐, 岡昌吾
  • Organizer: 第26回日本糖質学会年会
  • Place of Presentation: 仙台
  • Year and Date: 20060823-25
• [Presentation] Function and Regulation of the HNK-1 Carbohydrate in the Nervous System (2006)
  • Author(s): S. Oka, Y. Kizuka, S. Kakuda, I. Morita, S. Yamamoto and T. Kawasaki
  • Organizer: GlycoT 2006 5^<th> International Symposium on Glycosyltransferases
  • Place of Presentation: Tsukuba(Japan)
  • Year and Date: 20060625-28
• [Presentation] Structural and Biochemical Studies on Human Glucuronyltransferase, GlcAT-S (2006)
  • Author(s): I. Morita, T. Shiba, S. Kakuda, S. Oka, T. Kawasaki, S. Watatsuki, R. Kato
  • Organizer: Involved in the Biosynthesis of the Carbohydrate HNK-1.20^<th> IUBMB Inteanational Congress of Biochemistry and Molecular Biology and 11^<th> FAOBMB Congress
  • Place of Presentation: Kyoto(Japan)
  • Year and Date: 20060618-23
• [Presentation] A unique type of HNK-1 carbohydrate expressed on phosphacan is biosynthesized by GlcAT-P (2006)
  • Author(s): Y. Kizuka, N. Maeda, N. Kawasaki, T. Kawasaki, S. Oka
  • Organizer: 20^<th> IUBMB Inteanational Congress of Biochemistry and Molecular Biology and 11^<th> FAOBMB Congress
  • Place of Presentation: Kyoto(Japan)
  • Year and Date: 20060618-23
• [Presentation] メダカ胚発生に関わる糖転移酵素B4GALT2 の機能解析 (2006)
  • Author(s): 殿山泰弘, 安西大輔, 大上志穂, 川崎敏祐, 岡昌吾
  • Organizer: 日本発生生物学会第39回大会
  • Place of Presentation: 広島
  • Year and Date: 20060531-0603
• [Presentation] メダカを用いたHNK-1糖鎖の機能解析 (2006)
  • Author(s): 安西大輔, 殿山泰弘, 大上志穂, 川崎敏祐, 岡昌吾
  • Organizer: 日本発生生物学会第39回大会
  • Place of Presentation: 広島
  • Year and Date: 20060531-0603
• [Presentation] A traial with medaka (oryzias latipes) to analyze the function of glycans involved in the embryogenesis (2005)
  • Author(s): Y. Tonoyama, D. Anzai, S. Ogami and S. Oka
  • Organizer: 2nd Soul-Osaka-Kyoto Joint Symosium on Pharmaceutical Sciences
  • Place of Presentation: Osaka
  • Year and Date: 20051205-06
• [Presentation] メダカの発生における糖鎖の重要性 (2005)
  • Author(s): 岡昌吾
  • Organizer: 第28回日本分子生物学会
  • Place of Presentation: 福岡
  • Year and Date: 20051200
• [Presentation] Expression of the non-sulfated HNK-1 carbohydrate in mouse kidney (2005)
  • Author(s): S. Oka, H. Tagawa, Y. Kizuka, N. Kawasaki, H. Kurihara and T. Kawasaki
  • Organizer: Pacifichem 2005
  • Place of Presentation: Honolulu, Hawaii
  • Year and Date: 20051200
• [Book] Cell trace experiment with a caged fluorescent dye during medaka gastrulation in Medaka: Biology, Management, and Experimental Protocols. (M. Kinoshita, K. Murata, K. Naruse, M. Tanaka, Eds) (2009)
  • Author(s): Y. Tonoyama and S. Oka
  • Total Pages: (in press)
  • Publisher: Wiley-Blackwell
• [Book] Glucuronyltransferases Involved in the HNK-1 Biosynthesis. in Experimental Glycoscience-Glycobiology (N. Taniguchi, A. Suzuki, Y. Ito, H. Narimatsu, T. Kawasaki, and S. Hase, Eds) (2008)
  • Author(s): S. Kakuda, T. Kawasaki and S. Oka
  • Total Pages: pp47-49
  • Publisher: Springer
• [Book] Functional Roles of the HNK-1 Carbohydrate and Polysialic Acid in the Nervous System. in Experimental Glycoscience-Glycobiology (N. Taniguchi, A. Suzuki, Y. Ito, H. Narimatsu, T. Kawasaki, and S. Hase, Eds) (2008)
  • Author(s): Y. Kizuka and S. Oka
  • Total Pages: 180-181
  • Publisher: Springer
• [Book] Glucuronyltransferase (GlcAT-P) Gene-Deficient Mice. In Experimental Glycoscience-Glycobiology (N. Taniguchi, A. Suzuki, Y. Ito, H. Narimatsu, T. Kawasaki, and S. Hase, Eds) (2008)
  • Author(s): I. Morita and S. Oka
  • Total Pages: 381-382
  • Publisher: Springer
• [Remarks]
  • URL: http://www.med.kyoto-u.ac.jp/J/grad_school/introduction/5241/