2021 Fiscal Year Final Research Report

Establishment and re-establishment of uterine environment for pregnancy success

Research Project

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Project/Area Number	16H02584
Research Category	Grant-in-Aid for Scientific Research (A)
Allocation Type	Single-year Grants
Section	一般
Research Field	Animal production science
Research Institution	Tokai University (2018-2019, 2021) The University of Tokyo (2016-2017)
Principal Investigator	Imakawa Kazuhiko 東海大学, 総合農学研究所, 教授 (00291956)
Co-Investigator(Kenkyū-buntansha)	草間和哉東京薬科大学, 東京薬科大学薬学部, 助教 (30579149) 櫻井敏博東京理科大学, 薬学部薬学科, 助教 (70568253) 中尾洋一早稲田大学, 理工学術院, 教授 (60282696) 李俊佑東京大学, 大学院農学生命科学研究科(農学部), 助教 (10313082) 永岡謙太郎東京農工大学, (連合)農学研究科(研究院), 准教授 (60376564)
Project Period (FY)	2016-04-01 – 2020-03-31
Keywords	着床・胎盤形成 / 上皮間葉系転換 / 細胞融合 / トロホブラスト細胞 / 子宮内環境 / 受胎率向上 / 反芻動物
Outline of Final Research Achievements	In ruminants, trophoblast cells go through epithelial-mesenchymal transition (EMT) when implanting to the maternal endometrium. As EMT begins, trophoblast cells fuse each other to generate bi-nucleate or multi-nucleated cells, from which pregnancy associated glycoprotein is released. In addition, exosomes, miRNA and lncRNA were found from uterine flushing media and their functions were examined using in vitro uterine epithelial mono-culture or uterine epithelial-trophoblast co-culture systems. miRNA and lncRNA are effective in controling maternal immune system for semi-allogenic trophoblast cells.
Free Research Field	分子繁殖科学
Academic Significance and Societal Importance of the Research Achievements	この４年間、反芻動物の着床研究では世界をリードし続けることが出来た。特に、遺伝子・タンパク質発現及びエクソソーム・miRNAやlncRNA分野では先端の研究を続けるだけではなく論文を出し続けた。そのうちの1つ（業績論文9番目）のContinuous model of conceptus implantation to the maternal endometrium.はJ.Endocrinologyで世界で5番目にダウンロードされ、2017年、生殖分野で最も引用回数の多い論文となった。