Development of regenerative medicine for chronic pain: novel treatment with cell transplantation and drug delivery system.

2019 Fiscal Year Final Research Report

• Project/Area Number: 16H02678
• Research Category: Grant-in-Aid for Scientific Research (A)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Anesthesiology
• Research Institution: Gunma University
• Principal Investigator: SAITO SHIGERU 群馬大学, 大学院医学系研究科, 教授 (40251110)
• Co-Investigator(Kenkyū-buntansha): 荻野 祐一 群馬大学, 医学部附属病院, 講師 (20420094) ; 高澤 知規 群馬大学, 医学部附属病院, 講師 (30400766) ; 田畑 泰彦 京都大学, ウイルス・再生医科学研究所, 教授 (50211371) ; 須藤 貴史 群馬大学, 医学部附属病院, 講師 (60739621) ; 久保 和宏 群馬大学, 大学院医学系研究科, 助教 (80546531)
• Project Period (FY): 2016-04-01 – 2020-03-31
• Keywords: 慢性疼痛 / 再生医療 / 抑制ニューロン / 脊髄後角 / 神経障害性疼痛

Outline of Final Research Achievements

Pain and impaired endogenous analgesia are risk factors of chronic postsurgical persistent pain (CPSP). A Chronic neuropathic pain model induced by spinal nerve ligation shows impaired endogenous analgesia and delayed recovery from incisional pain. Repeated pharmacological treatment can restore the endogenous analgesia, but its effects on delayed recovery are not clear.
In this research project, several animal chronic painful practices including plantar incision were made on the side uni- and contralateral to the nerve ligation in chronic pain rats. Withdrawal thresholds were measured by pain assessment standards, such as von Frey filament test until 28 d after surgery. Among several tested drugs, amitriptyline (10 mg·kg-1·d-1) was considered effective for 13 d perioperatively. To examine the roles of noradrenergic and cholinergic signals in the spinal dorsal horn,

Free Research Field

麻酔科学

Academic Significance and Societal Importance of the Research Achievements

本研究により、末梢神経障害性の痛みでは下行性制御系の活動が重要である事が再確認されたことは痛みメカニズムの解明に寄与したと考えられる。また、抗てんかん薬、抗ケイレン薬系統の薬剤による慢性痛治療を更に有効にするための基礎データを得ることができた